NUCLEOTIDE-SEQUENCE ANALYSIS OF THE NUCLEOPROTEIN GENE OF AN AVIAN AND A HUMAN INFLUENZA-VIRUS STRAIN IDENTIFIES 2 CLASSES OF NUCLEOPROTEINS
NUCLEOTIDE-SEQUENCE ANALYSIS OF THE NUCLEOPROTEIN GENE OF AN AVIAN AND A HUMAN INFLUENZA-VIRUS STRAIN IDENTIFIES 2 CLASSES OF NUCLEOPROTEINS
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DOI：
10.1016/0042-6822(86)90198-4
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发表时间：
1986-12-01
期刊：
影响因子：
3.7
通讯作者：
MURPHY, BR
中科院分区：
文献类型：
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作者：
BUCKLERWHITE, AJ;MURPHY, BR
测定了一株禽流感A病毒A/绿头鸭/纽约/6750/78（H2N2）和一株人流感A病毒A/乌栋/307/72（H3N2）的RNA片段5的核苷酸序列，并将这些病毒核蛋白（NP）推导的氨基酸序列与另外两株禽流感和两株人流感A的NP序列进行了比较。结果表明，存在不同类别的禽流感A和人流感A的NP基因，可根据含禽流感和人流感病毒特异性氨基酸的位点以及氨基酸组成加以区分。人流感A病毒的NP基因似乎遵循一种线性进化途径，A/NT/60/68（H3N2）和A/乌栋/72之间的同源性最高（96.9%），这两株病毒仅相隔4年分离得到，而A/PR/8/34（H1N1）和A/乌栋/72之间的同源性最低（91.1%），它们相隔38年分离得到。此外，A/PR/8/34和A/NT/60/68之间84%的核苷酸替换在A/乌栋/72毒株的NP基因中得以保留。相比之下，禽流感的NP基因中不存在明显的线性进化途径，因为仅相隔5年分离得到的两株禽流感病毒A/鹦鹉/阿尔斯特/73（H7N1）和A/绿头鸭/78之间的同源性（90.3%）并不显著高于相隔44年分离得到的A/鸡瘟病毒/罗斯托克/34和A/绿头鸭/78之间的同源性（90.1%），而且A/鸡瘟病毒/34和A/鹦鹉/73之间只有49%的核苷酸替换在A/绿头鸭/78中存在。对人流感A病毒NP基因进化速率的测定表明，在1968年新亚型出现后的最初几年里，每年的核苷酸替换数量较多。
The nucleotide sequences of RNA segment 5 of an avian influenza A virus, A/Mallard/NY/6750/78 (H2N2), and a human influenza A virus, A/Udorn/307/72 (H3N2), were determined and the deduced amino acid sequences of the nucleoprotein (NP) of these viruses were compared to two other avian and two other human influenza A NP sequences. The results indicated that there are separate classes of avian and human influenza A NP gene that can be distinguished on the basis of sites containing amino acids specific for avian and human influenza viruses and also by amino acid composition. The human influenza A virus NP genes appear to follow a linear pathway of evolution with the greatest homology (96.9%) between A/NT/60/68 (H3N2) and A/Udorn/72, isolated only 4 years apart, and the least homology (91.1%) between A/PR/8/34 (H1N1) and A/Udorn/72, isolated 38 years apart. Furthermore, 84% of the nucleotide substitutions between A/PR/8/34 and A/NT/60/68 are preserved in the NP gene of the A/Udorn/72 strain. In contrast, a distinct linear pathway is not present in the avial influenza NP genes since the homology (90.3%) between the two avian influenza viruses A/Parrot/Ulster/73 (H7N1) and A/Mallard/78 isolated only 5 years apart is not significantly greater than the homology (90.1%) between strains A/FPV/Rostock/34 and A/Mallard/78 isolated 44 years apart and only 49% of the nucleotide substitutions between A/FPV/34 and A/Parrot/73 are found in A/Mallard/78. A determination of the rate of evolution of the human influenza A virus NP genes suggested that there were a greater number of nucleotide substitutions per year during the first several years immediately following the emergence of a new subtype in 1968.