Candida auris: a comparison between planktonic and biofilm susceptibility to antifungal drugs
Candida auris: a comparison between planktonic and biofilm susceptibility to antifungal drugs
复制标题
DOI：
10.1099/jmm.0.001036
复制
发表时间：
2019-09-01
影响因子：
3
通讯作者：
Esteban, Jaime
中科院分区：
文献类型：
--
作者：
Romera, David;Jairo Aguilera-Correa, John;Esteban, Jaime
导论.耳念珠菌是一种致病性酵母菌，主要影响免疫抑制患者和植入医疗器械的患者。这种病原体还表现出对常见抗真菌剂的高抗性以及高存活和传播能力。由于尚未确定该病原体的抗真菌折点，因此此处获得的数据可用于进一步研究预防和消毒方案的治疗或实施。本研究的目的是了解念珠菌的耐药性。耳对当前抗真菌药呈强直和固着状态。利用共聚焦激光扫描显微镜和可行的生物量生产，我们证明了C。形成成熟的生物膜。比较了不同浓度的最小抑菌浓度（MIC）和最小生物被膜清除浓度（MBEC）。auris DSM 21092菌株和两种临床分离株，并将结果与白色念珠菌和近平滑念珠菌（与血流感染和生物材料相关感染密切相关的两个菌种）的结果进行比较。我们发现临床分离的C.对氟康唑耐药，对棘白菌素和多烯类药物敏感。梭耳生物膜没有表现出对任何抗真菌剂的敏感性，显示MBEC高达512倍高于MICs.These研究结果强调了生物膜形成的重要性，作为一个关键因素，这种物种的抗真菌药物的耐药性，并建议植入式医疗设备的存在是免疫功能低下的患者的主要危险因素之一。
Introduction. Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this pathogen, the data obtained here can be useful for further research concerning treatment or implementation of a prevention and disinfection protocol. Our aim was to study the antifungal resistance of C. auris to current antifungals in planktonic and sessile states. Using confocal laser scanning microscopy and viable biomass production, we demonstrated the ability of C. auris to develop a mature biofilm. We compared the minimal inhibitory concentration (MIC) and the minimal biofilm eradication concentration (MBEC) for the C. auris DSM 21092 strain plus two clinical isolates, and the results were compared with those obtained for Candida albicans and Candida parapsilosis, two species strongly linked to bloodstream infections and infections associated with biomaterials. We found that the clinical isolates of C. auris were resistant to fluconazole and sensitive to echinocandins and polyenes. The C. auris biofilms did not show susceptibility to any antifungal agent, showing MBECs that were up to 512-fold higher than the MICs. These findings highlight the importance of biofilm formation as a key factor underlying the resistance of this species to antifungals and suggest that the presence of implantable medical devices is one of the major risk factors in immunocompromised patients.