Production of Calcium Maintenance Factor Stanniocalcin-1 (STC1) by the Equine Endometrium During the Early Pregnant Period
Production of Calcium Maintenance Factor Stanniocalcin-1 (STC1) by the Equine Endometrium During the Early Pregnant Period
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DOI：
10.1262/jrd.10-079k
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发表时间：
2011-04-01
影响因子：
1.8
通讯作者：
Imakawa, Kazuhiko
中科院分区：
文献类型：
--
作者：
Kikuchi, Masato;Nakano, Yasuko;Imakawa, Kazuhiko
一个因素负责进展到怀孕建立在母马尚未明确的特点。为了确定可能参与马妊娠建立的因素，从周期性第13天（第0天=排卵日）和第13、19和25天妊娠动物收集子宫内膜。从最初的消减杂交研究，钙调节因子，斯钙素-1（STC 1）mRNA，被发现作为候选分子表达独特的妊娠子宫内膜。子宫内膜STC 1 mRNA的表达在妊娠第19天，并在妊娠第25天的子宫内膜显着增加。STC 1蛋白在妊娠第25天子宫内膜的提取物中被发现，并且免疫化学定位在子宫腺中。此外，在从怀孕第25天的母马收集的子宫Hushing培养基中检测到STC 1蛋白。在第25天的妊娠中检测到高浓度的雌二醇-17 β（E-2）。妊娠子宫内膜中的E-2水平远高于其他区域，而不同子宫内膜区域的样本中孕酮水平没有差异。然而，在用不同浓度的E-2（0.01-100 ng/ml）处理的培养的子宫内膜外植体中，有或没有10 ng/ml的孕酮，STC 1 mRNA的表达没有显著上调。这些结果表明，增加STC 1的表达似乎符合胶囊消失的概念，并建议，STC 1从子宫腺可能发挥作用，在怀孕期间建立在母马的概念发展。
A factor responsible for progression to pregnancy establishment in the mare has not been definitively characterized. To identify factors possibly involved in the establishment of equine pregnancy, the endometrium was collected from day 13 (day 0=day of ovulation) cyclic and day 13, 19 and 25 pregnant animals. From initial subtractive hybridization studies, a calcium regulating factor, Stanniocalcin-1 (STC1) mRNA, was found as a candidate molecule expressed uniquely in the pregnant endometrium. Endometrial expression of STC1 mRNA was noted on day 19 and was markedly increased in the day 25 gravid endometrium. STC1 protein was found in the extracts of day 25 gravid endometrium and immunochemically localized in the uterine glands. In addition, STC1 protein was detected in uterine Hushing media collected from day 25 pregnant mares. High concentrations of estradiol-17 beta (E-2) were detected in day 25 conceptuses. E-2 levels were much higher in the gravid endometrium than in other regions, whereas progesterone levels did not differ among the samples from different endometrial regions. Expression of STC1 mRNA, however, was not significantly upregulated in cultured endometrial explants treated with various concentrations of E-2 (0.01-100 ng/ml) with or without 10 ng/ml progesterone. These results indicate that an increase in STC1 expression appears to coincide with capsule disappearance in the conceptus, and suggest that STC1 from the uterine glands likely plays a role in conceptus development during the pregnancy establishment period in the mare.