Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172
Full genomic analysis and possible origin of a porcine G12 rotavirus strain RU172
复制标题
DOI：
10.1007/s11262-010-0454-y
复制
发表时间：
2010-06-01
期刊：
影响因子：
1.6
通讯作者：
Naik, T. N.
中科院分区：
文献类型：
--
作者：
Ghosh, S.;Kobayashi, N.;Naik, T. N.
人A组轮状病毒（GAR）G12株被认为是急性胃肠炎的潜在重要病原体。另一方面，迄今为止，在动物中检测到G12的唯一报告是猪G12 P [7]菌株RU 172。菌株RU 172形成了与人G12菌株不同的单独的G12谱系，并且通过推导的氨基酸序列分析，具有猪源的VP 4、VP 6、NSP 4 -5。本研究测定了RU 172的VP 1、VP 3和NSP 1 -3基因的全长核苷酸序列以及VP 2基因的近全长核苷酸序列。通过核苷酸序列同源性和系统发育分析，将RU 172的VP 7-VP 4-VP 6-VP 1-VP 2-VP 3-NSP 1-NSP 2-NSP 3-NSP 4-NSP 5基因分别归属于G12-P[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1基因型。在其各自的基因型中，（i）RU 172的VP 1基因与Wa样人G12加尔斯的遗传相关性高于猪株，（ii）VP 2 -3和NSP 2基因与Wa样人（包括G12）和猪群分开聚类，而（iii）VP 6、NSP 1和NSP 3 -5基因与猪和猪样人群聚类。这些观察结果表明：（i）猪G12毒株可能起源于猪-人重配事件，或者（ii）Wa样人和猪G12毒株可能起源于共同的祖先，并最终随时间进化（通过遗传漂变和转移）。我们的研究结果为猪G12菌株的可能进化模式提供了重要的见解。
Human group A rotavirus (GAR) G12 strains are regarded as potentially important pathogens for acute gastroenteritis. On the other hand, to date, the only report of detection of G12 in animals was that of a porcine G12P[7] strain RU172. Strain RU172 formed a separate G12 lineage, distinct from human G12 strains, and by analyses of deduced amino acid sequences, had a VP4, VP6, NSP4-5 of porcine origin. In the present study, we determined the full-length nucleotide sequences of VP1, VP3, and NSP1-3 genes and nearly full-length nucleotide sequence of VP2 gene of RU172. By nucleotide sequence identities and phylogenetic analyses, the VP7-VP4-VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5 genes of RU172 were assigned to G12-P[7]-I5-R1-C1-M1-A1-N1-T1-E1-H1 genotypes, respectively. Within their respective genotypes, (i) VP1 gene of RU172 exhibited higher genetic relatedness to Wa-like human G12 GARs than porcine strains, (ii) VP2-3 and NSP2 genes clustered separately from the Wa-like human (including G12) and porcine clusters, while (iii) the VP6, NSP1 and NSP3-5 genes clustered with porcine and porcine-like human strains. These observations suggested that (i) the porcine G12 strain might have originated from porcine-human reassortment events, or alternatively, (ii) the Wa-like human and porcine G12 strains might have originated from a common ancestor, and eventually evolved (by genetic drift and shift) with time. Our findings provided important insights into the possible patterns of evolution of the porcine G12 strain.