Demonstration of macrophage chemotactic factors in the aqueous humor during experimental immunogenic uveitis in rabbits
Demonstration of macrophage chemotactic factors in the aqueous humor during experimental immunogenic uveitis in rabbits
复制标题
兔实验性免疫原性葡萄膜炎期间房水中巨噬细胞趋化因子的展示
作者：
N. Sher;K. Foon;M. Fishman;T. Brown
在实验性兔葡萄膜炎中，体液中的因子能够增加单核细胞的随机迁移和趋化性。将无内毒素的人血清白蛋白注射到玻璃体中，7天后可检测到严重炎症，12 - 21天最严重。用改良的Boyden室测量房水的趋化活性，该Boyden室允许使用小于0.025 ml的体积。化学活性在原发性炎症期间是可测量的，并且在临床炎症和前房细胞反应减少后的短时间内保持升高。30天后静脉注射人血清白蛋白可引发严重的再诱导葡萄膜炎。趋化因子可以测量高滴度后8小时，这静脉内的挑战。
Factors in the queous humor capable of increasing the random migration and chemotaxis of monocytes were demonstrated during experimental uveitis in the rabbit. Injection of endotoxin-free human serum albumin into the vitreous produced a severe inflammation detectable after 7 days and maximal between 12 to 21 days. Aqueous humor was measured for chemotactic activity with a modified Boyden chamber, which permitted the use of volumes of less than 0.025 ml. Chematactic activity was measurable during the primary inflammation and remained elevated for a short period of time after clinical inflammation and anterior chamber cellular reaction had decreased. A severe reinduction uveitis could be initiated by the intravenous injection of human serum albumin after day 30. Chemotactic factors could be measured at high titers by 8 h after this intravenous challenge.