Intracellular Delivery of Nanoparticles with Cell Penetrating Peptides
Intracellular Delivery of Nanoparticles with Cell Penetrating Peptides
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DOI：
10.1007/978-1-4939-2806-4_24
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发表时间：
2015-01-01
期刊：
影响因子：
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通讯作者：
Torchilin, Vladimir P.
中科院分区：
文献类型：
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作者：
Salzano, Giuseppina;Torchilin, Vladimir P.
用细胞穿透肽（CPP）功能化纳米颗粒（NP）构成了治疗和诊断有效载荷的细胞内递送的突破。在1998年底，观察到细胞对来自HIV-1病毒的一种小蛋白，即达特肽（TATp）的显著摄取。此后，研究开始设计类似作用的肽，并将NP与这些新的CPP偶联。在这里，我们描述了最近的方法，用于修改与CPPs和在体外和体内的影响，这种功能化的各种货物的细胞内交付的纳米粒子的表面。特别是，我们强调了最近的进展，旨在减少非选择性的CPP和防止其酶裂解途中的靶组织。
The functionalization of nanoparticles (NPs) with cell penetrating peptides (CPPs) constitutes a breakthrough for the intracellular delivery of therapeutic and diagnostic payloads. In late 1998, a significant cellular uptake of a small protein from the HIV-1 virus, namely TAT peptide (TATp), was observed. Thereafter, research began on design of similarly acting peptides, and the coupling of NPs with these novel CPPs. Here, we describe recent methods used to modify the surface of NPs with CPPs and the in vitro and in vivo effects of such functionalization on the intracellular delivery of various cargos. In particular, we highlight recent advances aimed at reducing the non-selectivity of CPPs and the prevention of their enzymatic cleavage en route to target tissues.