Liposome Enhanced Detection of Amyloid Protein Aggregates.
Liposome Enhanced Detection of Amyloid Protein Aggregates.
复制标题
脂质体增强淀粉样蛋白聚集体的检测。
DOI：
10.1021/acs.orglett.0c03597
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发表时间：
2021
期刊：
影响因子：
5.2
通讯作者：
Kocsis I
中科院分区：
文献类型：
--
作者：
Kocsis I
巯基黄素- t用于淀粉样蛋白聚集体成像，因为它具有优异的开启荧光特性，但结合亲和力较低。通过在囊泡表面安装多个染料单元，对α-突触核蛋白原纤维的结合亲和力提高了3个数量级，光学响应增强。染料头基团和脂质与蛋白质的合作相互作用为基于囊泡的多价淀粉样蛋白探针的构建提供了一般策略。
Thioflavin-T is used to image amyloid aggregates because of the excellent turn-on fluorescence properties, but binding affinities are low. By mounting multiple dye units on the surface of a vesicle, the binding affinity for α-synuclein fibrils is increased by three orders of magnitude, and the optical response is increased. Cooperative interactions of the dye headgroup and lipid with the protein provide a general strategy for the construction of multivalent amyloid probes based on vesicles.