Human placenta metabolizes fatty acids: implications for fetal fatty acid oxidation disorders and maternal liver diseases
Human placenta metabolizes fatty acids: implications for fetal fatty acid oxidation disorders and maternal liver diseases
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DOI：
10.1152/ajpendo.00481.2002
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发表时间：
2003-06-01
影响因子：
5.1
通讯作者：
Strauss, AW
中科院分区：
文献类型：
--
作者：
Shekhawat, P;Bennett, MJ;Strauss, AW
人类妊娠期间脂肪代谢的作用以及对胎盘生长和功能的影响还知之甚少。受影响胎儿的线粒体脂肪酸氧化障碍与妊娠母体疾病有关，包括先兆子痫、妊娠急性脂肪肝、溶血、肝酶升高和称为HELLP的血小板减少综合征。我们研究了六种脂肪酸β-氧化酶在不同胎龄的人胎盘中的发育表达和活性。免疫组织化学和免疫印迹分析显示，胎盘标本中所有六种酶都有丰富的表达，与其他类型的胎盘细胞相比，合体滋养层细胞的染色更强。胎盘组织中的β-氧化酶活性在妊娠早期较高，近期较低。滋养层细胞在培养中大量氧化氚标记的棕榈酸酯和肉豆蔻酸酯，表明人类胎盘利用脂肪酸作为重要的代谢燃料。因此，人类胎盘从脂肪酸氧化中获得能量，为胎儿脂肪酸氧化障碍与怀孕期间母亲肝脏疾病的关联提供了一个潜在的解释。
The role of fat metabolism during human pregnancy and in placental growth and function is poorly understood. Mitochondrial fatty acid oxidation disorders in an affected fetus are associated with maternal diseases of pregnancy, including preeclampsia, acute fatty liver of pregnancy, and the hemolysis, elevated liver enzymes, and low platelets syndrome called HELLP. We have investigated the developmental expression and activity of six fatty acid beta-oxidation enzymes at various gestational-age human placentas. Placental specimens exhibited abundant expression of all six enzymes, as assessed by immunohistochemical and immunoblot analyses, with greater staining in syncytiotrophoblasts compared with other placental cell types. beta-Oxidation enzyme activities in placental tissues were higher early in gestation and lower near term. Trophoblast cells in culture oxidized tritium-labeled palmitate and myristate in substantial amounts, indicating that the human placenta utilizes fatty acids as a significant metabolic fuel. Thus human placenta derives energy from fatty acid oxidation, providing a potential explanation for the association of fetal fatty acid oxidation disorders with maternal liver diseases in pregnancy.