Determining the Optimal Storage Time and Temperature for Performing Platelet Function Assays and Global Hemostasis Assays
Determining the Optimal Storage Time and Temperature for Performing Platelet Function Assays and Global Hemostasis Assays
复制标题
DOI：
10.1080/09537104.2021.1934666
复制
发表时间：
2021-06-13
期刊：
影响因子：
3.3
通讯作者：
Henskens, Y. M. C.
中科院分区：
文献类型：
--
作者：
Denessen, E. J. S.;Van den Kerkhof, D. L.;Henskens, Y. M. C.
血小板功能测定和整体止血测定对于诊断出血倾向至关重要，其中光透射聚集度测定 (LTA) 是金标准。多电极聚集（Multiplate）、血小板功能测定（PFA）和旋转血栓弹力测定（ROTEM）主要用作全血筛查测试。目前，由于需要特定的专业知识，患者必须前往专门的实验室进行这些测试。分析前的变量，如储存时间和运输过程中的温度，仍然被认为是过程中最脆弱的部分，可能会导致测试结果的差异。我们的目的是对健康志愿者血液样本在储存过程中的稳定性产生第一印象，并研究储存时间（1、3、6 和 24 小时）和温度（4 摄氏度、室温和 37 摄氏度）对 Multiplate、PFA、ROTEM 和 LTA 测试结果的影响。我们的数据表明，对于 PFA，全血可以在室温下保存 3 小时。用于 Multiplate 和 ROTEM 的全血可保存 6 小时。对于 LTA，PRP 和全血分别在 4 摄氏度或室温下稳定 3 小时，在室温下稳定 6 小时。
Platelet function assays and global haemostasis assays are essential in diagnosing bleeding tendencies, with light transmission aggregometry (LTA) as golden standard. The Multiple Electrode Aggregation (Multiplate), platelet function assay (PFA) and rotational thromboelastometry (ROTEM) are mostly used as whole-blood screening tests. Currently, patients have to travel to specialized laboratories to undergo these tests, since specific expertise is required. Pre-analytical variables, like storage time and temperature during transport, are still considered to be the most vulnerable part of the process and may lead to discrepancies in the test results. We aim to give a first impression on the stability of blood samples from healthy volunteers during storage and investigate the effect of storage time (1, 3, 6 and 24 hours) and temperature (4 degrees C, room temperature and 37 degrees C) on the Multiplate, PFA, ROTEM and LTA test results. Our data indicated that, for the PFA, whole blood can be stored for 3 hours at room temperature. Whole blood used for the Multiplate and ROTEM can be stored for 6 hours of storage. For LTA, PRP and whole blood were stable up to 3 hours at 4 degrees C or room temperature and 6 hours at room temperature, respectively.