Co‐incident N and K ras gene mutations in a case of AML, restricted to differing cell lineages
Co‐incident N and K ras gene mutations in a case of AML, restricted to differing cell lineages
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AML 病例中同时发生的 N 和 K ras 基因突变，仅限于不同的细胞谱系
DOI：
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发表时间：
1989
影响因子：
6.5
通讯作者：
B. Young
中科院分区：
文献类型：
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作者：
L. Hiorns;F. Cotter;B. Young
来自M5 FAB分类的急性髓性白血病（AML）患者的外周血显示具有N和K ras基因突变。在不连续Percoll密度梯度上分离白细胞化的血液以提供富集不同细胞谱系的级分。使用聚合酶链反应（PCR）技术扩增从这些馏分中提取的DNA，并与先前证明的单碱基突变特异性的寡核苷酸探针杂交。N-ras突变仅限于原始细胞和单核细胞部分，与M5的FAB亚型一致。然而，K-ras突变存在于所有组分中，表明它发生在多潜能干细胞中，代表白血病产生的早期阶段，或可能是偶然的背景现象。
Peripheral blood from a patient with acute myeloid leukaemia (AML) of M5 FAB classification, was shown to have mutations to both the N and K ras genes. Leucophoresed blood was separated on a discontinuous Percoll density gradient to provide fractions enriched for different cell lineages. DNA extracted from these fractions was amplified using the polymerase chain reaction (PCR) technique, and hybridized with oligonucleotide probes specific for the single base mutations previously demonstrated. The N‐ras mutation was shown to be restricted to the blast and monocytic cell fractions, concordant with the FAB subtype of M5. The K‐ras mutation, however, was present in all fractions, suggesting it had occurred in a multi‐potential stem cell representing an earlier stage in the generation of the leukaemia, or possibly an incidental background phenomenon.
影响因子：
16.6
作者：
M. Barbacid
通讯作者：
M. Barbacid