Measurement of Chlorophyll Fluorescence as a Heat Stress Indicator in Tomato: Laboratory and Greenhouse Comparisons
Measurement of Chlorophyll Fluorescence as a Heat Stress Indicator in Tomato: Laboratory and Greenhouse Comparisons
复制标题
叶绿素荧光测量作为番茄热应激指标：实验室和温室比较
DOI：
10.21273/jashs.126.2.188
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
M. Peet
中科院分区：
文献类型：
--
作者：
D. Willits;M. Peet
在实验室和温室条件下测量叶绿素荧光，以努力开发能够预测番茄（Lycoper-sicon esculentum Mill.）在温室条件下。实验室测试包括测量可变叶绿素荧光与最大叶绿素荧光的比率（Fv/Fm），所述叶绿素荧光在从整个番茄叶中取出的叶盘上进行，并放置在温度控制板上。进行了比较与温室测量的相同参数进行完整的叶片暴露于不同的温度处理施加的空气环境的温室的空气处理的整个植物。暗适应期范围从15分钟到整天在温室和温度暴露期范围从5分钟到60分钟在实验室进行了比较，以找到两个测试之间的最佳相关性。实验室处理时间为60 min，温室暗适应时间为60 min，获得了最佳一致性。Fv/Fm下降二次叶温以类似的方式在这两个测试，表明实验室的方法可以充分预测植物对温室热胁迫的反应。
Chlorophyll fluorescence was measured under both laboratory and greenhouse conditions in an effort to develop a quick, reliable, and inexpensive laboratory procedure capable of predicting heat stress experienced by tomato ( Lycoper- sicon esculentum Mill.) under greenhouse conditions. The laboratory tests consisted of measurements of the ratio of variable to maximal chlorophyll fluorescence (F v/Fm) performed on leaf discs taken from whole tomato leaves and placed on a temperature controlled plate. Comparisons were made with greenhouse measurements of the same parameter conducted on intact leaves of whole plants exposed to different temperature treatments imposed by manipulation of the aerial environment of the greenhouse. Dark adaption periods ranging from 15 min to all day in the greenhouse and temperature exposure periods ranging from 5 min to 60 min in the laboratory were compared to find the best correlation between the two tests. Best agreement was obtained with 60 min treatment times in the laboratory and 60 min dark adaption periods in the greenhouse. Fv/Fm decreased quadratically with increasing leaf temperature in a similar fashion in both tests, suggesting that the laboratory approach can adequately predict plant response to greenhouse heat stress.