Responses to influenza vaccination in different T-cell subsets: a comparison of healthy young and older adults
Responses to influenza vaccination in different T-cell subsets: a comparison of healthy young and older adults
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DOI：
10.1016/s0264-410x(98)00133-9
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发表时间：
1998-11-01
期刊：
影响因子：
5.5
通讯作者：
Meneilly, GS
中科院分区：
文献类型：
--
作者：
McElhaney, JE;Upshaw, CM;Meneilly, GS
在健康的年轻人和老年人志愿者中测量了对流感疫苗接种的T淋巴细胞应答。所有参与者均接种了1995-96年三价流感疫苗。在病毒刺激后、接种前、接种后4周和12周，在外周血单核细胞（PBMC）培养物中测定细胞因子和颗粒酶B水平。老年人组的主要发现是对每种疫苗病毒株的不同类型的辅助T细胞（T-h）反应和对疫苗接种的极差细胞毒性T淋巴细胞（通过颗粒酶B测量）反应。IL-10是在T-h-2型应答中产生的，在A/Texas/36/91（H1N1）刺激的PBMC中比A/Johannesburg/33/94（H3 N2）刺激的PBMC中更高;与年轻人相比，老年人的PBMC中这种差异更显著。相比之下，IL-2，这是在T-h-1型反应中产生的，在相同的培养物中进行了测量，并显着高于A/约翰内斯堡/33/94刺激的PBMC。IFN-γ水平在用B/Harbin/7/94刺激的PBMC中最高。最大的年龄相关性差异是颗粒酶B的水平在所有病毒刺激的PBMC从年轻人与老年人组相比。疫苗中所含的流感病毒株以及受试者的年龄似乎是T细胞对疫苗接种反应的非常重要的决定因素。(C)1998爱思唯尔科技有限公司版权所有。
T-lymphocyte responses to influenza vaccination were measured in healthy young and older adult volunteers. All participants were vaccinated with the 1995-96 trivalent influenza vaccine. Cytokine and granzyme B levels were measured in peripheral blood mononuclear cells (PBMC) cultures after virus stimulation, prior to and 4 and 12 weeks after vaccination. The major findings in the older adult group were the different types of helper T-cell (T-h) responses to each of the vaccine strains of virus and a very poor cytotoxic T lymphocyte (as measured by granzyme B) response to vaccination. IL-10, which is produced in a T-h-type 2 response, was higher in PBMC stimulated with A/Texas/36/91 (H1N1) compared with A/Johannesburg/33/94 (H3N2); this difference was more marked in the PBMC from older compared with younger adults. In contrast, IL-2, which is produced in a T-h-type 1 response, was measured in the same cultures and was significantly higher in A/Johannesburg/33/94-stimulated PBMC. IFN-gamma levels were highest in the PBMC stimulated with B/Harbin/7/94. The greatest age-related difference was the level of granzyme B in all virus-stimulated PBMC from the young compared with the older adult group. The strain of influenza virus contained in the vaccine, as well as the age of the subject, appear to be very important determinants of the T-cell response to vaccination. (C) 1998 Elsevier Science Ltd. All rights reserved.