A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible
A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible
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DOI：
10.1016/j.jplph.2005.04.031
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发表时间：
2006-03-01
影响因子：
4.3
通讯作者：
Tang, KX
中科院分区：
文献类型：
--
作者：
Liu, XJ;Huang, BB;Tang, KX
从黄果茄（Solanumsurattense）中分离并鉴定了编码PR 10蛋白的cDNA克隆（命名为SsPR 10，GenBank登录号AY 660753）。SsPR 10基因编码一个160个氨基酸的多肽，预测分子量为17.58 kDa，pI为5.29。序列比对结果表明，SsPR 10与CaPR 10的同源性为68.1%，而与JIOsPR 10的同源性仅为31.7%。基因组DNA印迹分析表明，SsPR 10属于一个多基因家族。检测到组成型表达的SsPR 10在罐子培养的无菌幼苗的根中表达最高，而SsPR 10在用含有TMV的汁液孵育的幼苗的老黄叶中表达最高。SsPR 10在衰老叶片中的表达水平始终略高于幼嫩叶片。进一步的表达分析表明，防御/胁迫途径的信号组分（MeJA、SA、阿坝、GA（3）、H2 O2和Cu 2+）显著上调了SsPR 10的mRNA水平。然而，黑暗不能诱导SsPR 10的表达，其表达也被冷处理抑制。成功地在大肠杆菌中表达了SsPR 10，并对表达产物进行了纯化。透析复性的SsPR 10蛋白没有磷酸化，表现出对S.对梨孢霉菌丝生长有抑制作用。我们的研究结果表明，新的胁迫和病原体诱导的SsPR 10与核糖核酸裂解和抗菌活性不仅参与防御/胁迫反应途径，而且还参与植物的生长，发育和衰老。(C)2005年Elsevier GmbH。All rights reserved.
A cDNA clone (designated as SsPR10, GenBank Accession Number AY660753) encoding a PR10 protein from yellow-fruit nightshade (Solanum surattense) was isolated and characterized. SsPR10 encoded a 160-amino-acid potypeptide with a predicted molecular mass of 17.58 kDa and pl of 5.29. Sequence alignments showed that SsPR10 had high identity (68.1%) with CaPR10, but had only about 31.7% identity with JIOsPR10 at the amino acid level. Genomic DNA get blot analysis indicated that SsPR10 belonged to a multigene family. The constitutively expressed SsPR10 was detected to be the highest in roots of the sterile seedlings cultured in jars, while SsPR10 expression was the highest in old yellow leaves from the seedlings incubated with sap containing TMV. SsPR10 always expressed at slightly higher level in senescent leaves than in tender ones under both conditions. Further expression analysis revealed that the signaling components of defense/stress pathways (MeJA, SA, ABA, GA(3), H2O2 and Cu2+) up-regulated significantly the SsPR10 mRNA levels over the control. However, darkness failed to induce SsPR10 expression and its expression was also inhibited by cold treatment. The SsPR10 was successfully expressed in Eschericha coli and the expressed protein was purified to near homogeneity. The dialytically renatured SsPR10 protein without phosphorylation exhibited ribonucleolytic activity against S. surattense leaf total RNA preparations and could inhibit hyphal growth of Pyricularia oryzae. Our findings suggest that the novel stress- and pathogen-inducible SsPR10 with ribonucleolytic and antimicrobial activity participates not only in the defense/stress response pathways but also in plants' growth, development and senescence. (C) 2005 Elsevier GmbH. All rights reserved.