GENETICAL AND STRUCTURAL ANALYSES OF CELL-WALL FORMATION IN CHLAMYDOMONAS-REINHARDI
GENETICAL AND STRUCTURAL ANALYSES OF CELL-WALL FORMATION IN CHLAMYDOMONAS-REINHARDI
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DOI：
10.1017/s0016672300012015
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发表时间：
1971-01-01
期刊：
影响因子：
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通讯作者：
PLASKITT, A
中科院分区：
文献类型：
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作者：
DAVIES, DR;PLASKITT, A
本文分离了15株莱茵衣藻突变株，它们在细胞壁形成过程的某些方面存在缺陷。遗传分析表明，大多数突变是由单基因改变引起的；其中两个异常是在与其他基因型杂交时获得非孟德尔分离，而在自交时，它们经常产生野生型表型。从这两个菌株中也偶有获得体细胞回复性。在这些分析的基础上，我们提出细胞壁生物发生过程中有两个层次的控制，一个与核水平上的亚基产生有关，另一个可能与三维组织有关。对不同突变体的电子显微镜分析显示，突变体可分为三大类：细胞壁形成但不附着在质膜上的突变体，细胞壁附着在膜上的突变体，以及细胞壁很少形成的突变体。特别是在上节课中，我们可以清楚地看到含有壁前体的囊泡，它们通过质膜脱落到培养基中。
Fifteen mutant strains of Chlamydomonas reinhardi were isolated which showed defects in some aspect of the process of cell-wall formation. Genetic analyses indicated that most of the mutations were due to single gene changes; two were anomalous in that non-Mendelian segregations were obtained on crossing with other genotypes, and on selfing they frequently gave rise to wild-type phenotypes.Occasional somatic revertants were also obtained from these two strains. On the basis of these analyses it is suggested that there are two levels of control operating in the process of cell wall biogenesis - one concerned with subunit production at the nuclear level and another, possibly concerned with three-dimensional organization, at another level. Electron-microscope analyses of the different mutants showed the mutants to be divided into three main categories: those in which the wall was formed but was not attached to the plasma membrane, those in which the wall was attached to the membrane, and those in which very little wall was formed. In the last class in particular, vesicles containing wall precursors were clearly visible, and were shed through the plasma membrane into the medium.