Intrahepatic triglyceride measurement and estimation of viability in rat fatty livers by near-infrarcd spcctroscopy.
Intrahepatic triglyceride measurement and estimation of viability in rat fatty livers by near-infrarcd spcctroscopy.
复制标题
通过近红外光谱法测量肝内甘油三酯并评估大鼠脂肪肝的活力。
DOI：
10.1111/hepr.12364
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发表时间：
2015
影响因子：
4.2
通讯作者：
Ohdan H.
中科院分区：
文献类型：
--
作者：
Fudaba Y;Oshita A (First co-author);Tashiro H;Ohdan H.
目的本研究利用近红外（NIR）光谱研究了脂肪肝的特征，并验证了近红外光谱在测量肝内甘油三酯（TG）含量和评估脂肪肝活力方面的有用性。方法在体外，我们通过近红外检查了每种纯化的TG组分的特定光谱。在体内，对热缺血前从正常和脂肪肝获得的光谱之间的差异以及从热缺血前后从每只大鼠肝脏获得的光谱之间的差异进行了研究结果体外实验显示主要TG组分在925nm处有一个特异峰，近红外光谱精确检测到TG体积的变化。体内实验显示，在最小二乘曲线拟合中添加纯化的TG光谱进行近红外光谱分析后，近红外光谱检测到缺乏胆碱饮食诱导的大鼠脂肪肝中TG含量的变化。通过近红外光谱法测量的脂肪肝中的甘油三酯水平与肝脏中脂质含量的形态测量显着相关。近红外光谱还揭示了正常条件下脂肪肝中总血红蛋白 (Hb) 和氧化 Hb 水平的降低以及细胞色素氧化还原状态的稳态维持。然而，近红外光谱显示，在60分钟的热缺血再灌注后，脂肪肝中的肝微循环、血红蛋白氧合和细胞色素氧化还原状态的稳态出现不可逆的恶化。结论这些研究表明，近红外光谱除了同时评估微循环和血红蛋白氧合之外，还可以定量测量肝内TG含量。
AimThis study investigated the features of fatty livers using near‐infrared (NIR) spectroscopy and validated the usefulness of NIR spectroscopy for the measurement of intrahepatic triglyceride (TG) contents and evaluation of viability in fatty livers.MethodsIn vitro, we examined specific spectra for each purified TG fraction by NIR.In vivo, the differences between the spectra obtained from normal and fatty livers before warm ischemia and the differences between the spectra obtained from each rat liver before and after warm ischemia were subjected to multicomponent analysis.ResultsIn vitroexperiments revealed a specific peak at 925 nm in major TG fractions, and NIR spectroscopy precisely detected changes in TG volume.In vivoexperiments revealed that NIR spectroscopy detected TG content changes in rat fatty livers induced by a choline‐deficient diet following the addition of purified TG spectrum for NIR spectroscopic analysis in least square curve fitting. The TG level in the fatty livers measured by NIR spectroscopy significantly correlated with the morphometric measurement of lipid content in the livers. NIR spectroscopy also revealed decreased levels of total hemoglobin (Hb) and oxidized Hb and maintenance of homeostasis in cytochrome redox states in fatty livers under normal condition. However, NIR spectroscopy showed irreversible deterioration of hepatic microcirculation, Hb oxygenation and homeostasis of the cytochrome redox states in fatty livers after 60‐min warm ischemia reperfusion.ConclusionThese studies demonstrated that NIR spectroscopy can quantitatively measure the intrahepatic TG content in addition to simultaneously evaluating microcirculation and Hb oxygenation.