Role of a Tannerella forsythia exopolysaccharide synthesis operon in biofilm development
Role of a Tannerella forsythia exopolysaccharide synthesis operon in biofilm development
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DOI：
10.1016/j.micpath.2007.01.003
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发表时间：
2007-04-01
影响因子：
3.8
通讯作者：
Sharma, Ashu
中科院分区：
文献类型：
--
作者：
Honma, Kiyonobu;Inagaki, Satoru;Sharma, Ashu
Tannerella pasthia是一种革兰氏阴性口腔厌氧菌，与牙周炎的发展有关，牙周炎是一种由细菌感染引起的慢性炎症性疾病，如果不治疗会导致牙齿脱落。由于牙周细菌形成的生物膜被认为是疾病进展的重要因素，并在治疗中造成困难，我们试图研究的根本机制，结核菌bjofilm形成。这是通过筛选T.生物膜发育的改变。这种方法导致参与胞外多糖（EPS）合成的操纵子的鉴定。构建了操纵子内所含的基因之一wecC的同基因突变体。与亲本菌株相比，同基因的wecC突变体显示出增加的形成生物膜的能力。的wecC突变体也形成聚集的小菌落，并显示出增加的细胞表面相关的疏水性相比，亲本菌株。此外，wecC突变体的生化表征表明，表面糖蛋白的糖基化减少。因此，我们的研究结果表明，wecC操纵子与表面糖蛋白表达的糖基化有关，并可能在T.微生物膜形成。(c)2007爱思唯尔有限公司保留所有权利。
Tannerella forsythia is a Gram-negative oral anaerobe implicated in the development of periodontitis, a chronic inflammatory disease induced by bacterial infections which leads to tooth loss if untreated. Since biofilms formed by periodontal bacteria are considered important in disease progression and pose difficulties in treatment, we sought to investigate the underlying mechanisms of T forsythia bjofilm formation. This was carried out by screening random insertion mutants of T. forsythia for alterations in biofilm development. This approach lead to the identification of an operon involved in exopolysaccharide (EPS) synthesis. An isogenic mutant of one of the genes, wecC, contained within the operon was constructed. The isogenic wecC mutant showed increased ability to form biofilms as compared to the parent strain. The wecC mutant also formed aggregated microcolonies and showed increased cell-surface associated hydrophobicity as compared to the parent strain. Moreover, biochemical characterization of the wecC mutant indicated that glycosylation of surface glycoproteins was reduced. Therefore, our results Suggest that the wecC operon is associated with glycosylation of surface-glycoprotein expression and likely plays an inhibitory role in T. forsythia biofilm formation. (c) 2007 Elsevier Ltd. All rights reserved.