First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association
First isolation of microorganisms from the gut diverticulum of Aedes aegypti (Diptera: Culicidae): new perspectives for an insect-bacteria association
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DOI：
10.1590/s0074-02762007000800005
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发表时间：
2007-12-01
期刊：
影响因子：
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通讯作者：
Lemos, Francisco José Alves
中科院分区：
文献类型：
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作者：
Gusmão, Desiely Silva;Santos, Adão Valmir;Lemos, Francisco José Alves
我们首次表明，蚊子肠道（不渗透的糖储存器官）的腹侧憩室窝藏微生物。在无菌条件下解剖来自新出现的和未进食的埃及伊蚊的肠憩室，匀浆化并铺在BHI培养基上。通过对细菌的16S rDNA和酵母的28S rDNA进行测序来鉴定微生物分离株。从Ae.还进行了埃及肠憩室。细菌分离物是：芽孢杆菌属，枯草芽孢杆菌和沙雷氏菌属。后者是我们的分离株中发现的优势细菌。鉴定的酵母种是加勒比毕赤酵母。
We show for the first time that the ventral diverticulum of the mosquito gut (impermeable sugar storage organ) harbors microorganisms. The gut diverticulum from newly emerged and non-fed Aedes aegypti was dissected under aseptic conditions, homogenized and plated on BHI medium. Microbial isolates were identified by sequencing of 16S rDNA for bacteria and 28S rDNA for yeast. A direct DNA extraction from Ae. aegypti gut diverticulum was also performed. The bacterial isolates were: Bacillus sp., Bacillus subtilis and Serratia sp. The latter was the predominant bacteria found in our isolations. The yeast species identified was Pichia caribbica.