Insights into the Structure of Dimeric RNA Helicase CsdA and Indispensable Role of Its C-Terminal Regions.
Insights into the Structure of Dimeric RNA Helicase CsdA and Indispensable Role of Its C-Terminal Regions.
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深入了解二聚体 RNA 解旋酶 CsdA 的结构及其 C 末端区域的不可或缺的作用。
DOI：
10.1016/j.str.2017.09.013
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发表时间：
2017-12
期刊：
影响因子：
5.7
通讯作者：
Shi Yunyu
中科院分区：
文献类型：
--
作者：
Xu Ling;Wang Lijun;Peng Junhui;Li Fudong;Wu Lijie;Zhang Beibei;Lv Mengqi;Zhang Jiahai;Gong Qingguo;Zhang Rongguang;Zuo Xiaobing;Zhang Zhiyong;Wu Jihui;Tang Yajun;Shi Yunyu
CSDA被认为在冷休克后核糖体的生物发生和基因调控中是必不可少的。然而，CSDA的结构及其C-端长区域的功能仍不清楚。在这里，我们解析了CSDA的所有结构域，并发现了两个以前未描述的辅助结构域：二聚化结构域(DD)和RNA结合结构域(RBD)。小角X射线散射实验有助于跟踪解旋酶核心区和C-末端区域的构象柔性。生化分析表明，DD对于稳定CSDA二聚体结构是必不可少的。我们还首次证明了CSDA在低温下是一种稳定的二聚体。C-末端区域对于RNA结合和有效的酶活性是至关重要的。CSDA_RBD可以特异性地结合偏爱单链G-丰富RNA的区域，这可能有助于使解旋酶核心解开相邻的双链。
CsdA has been proposed to be essential for the biogenesis of ribosome and gene regulation after cold shock. However, the structure of CsdA and the function of its long C-terminal regions are still unclear. Here, we solved all of the domain structures of CsdA and found two previously uncharacterized auxiliary domains: a dimerization domain (DD) and an RNA-binding domain (RBD). Small-angle X-ray scattering experiments helped to track the conformational flexibilities of the helicase core domains and C-terminal regions. Biochemical assays revealed that DD is indispensable for stabilizing the CsdA dimeric structure. We also demonstrate for the first time that CsdA functions as a stable dimer at low temperature. The C-terminal regions are critical for RNA binding and efficient enzymatic activities. CsdA_RBD could specifically bind to the regions with a preference for single-stranded G-rich RNA, which may help to bring the helicase core to unwind the adjacent duplex.
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影响因子：
14.9
作者：
Steimer L;Wurm JP;Linden MH;Rudolph MG;Wöhnert J;Klostermeier D
通讯作者：
Klostermeier D
影响因子：
4.5
作者：
M. Rudolph;D. Klostermeier
通讯作者：
M. Rudolph;D. Klostermeier
DOI：
10.1002/prot.20864
发表时间：
2006-03
期刊：
Proteins: Structure
影响因子：
--
作者：
J. Tsai;Bo Chen;Hui-Chun Cheng;Hsin-Yi Chen;Nai-Wan Hsaio;P. Lyu;Yuh-Ju Sun
通讯作者：
J. Tsai;Bo Chen;Hui-Chun Cheng;Hsin-Yi Chen;Nai-Wan Hsaio;P. Lyu;Yuh-Ju Sun
影响因子：
2.9
作者：
Bizebard, T;Ferlenghi, I;Dreyfus, M
通讯作者：
Dreyfus, M
影响因子：
6.1
作者：
Konarev, PV;Volkov, VV;Svergun, DI
通讯作者：
Svergun, DI