Interleukin-6 Increases Intracellular Ca2+ Concentration and Induces Catecholamine Secretion in Rat Carotid Body Glomus Cells
Interleukin-6 Increases Intracellular Ca2+ Concentration and Induces Catecholamine Secretion in Rat Carotid Body Glomus Cells
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Interleukin-6 增加大鼠颈动脉体球细胞细胞内 Ca2 浓度并诱导儿茶酚胺分泌
DOI：
10.1002/jnr.22107
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发表时间：
2009-09-01
影响因子：
4.2
通讯作者：
Wang, Bai-Ren
中科院分区：
文献类型：
--
作者：
Fan, Juan;Zhang, Bo;Wang, Bai-Ren
尽管大量证据表明免疫系统与中枢神经系统之间存在相互调节，但免疫信号如何传递到大脑仍然是一个悬而未决的问题。在先前的研究中，我们发现了促炎细胞因子受体的强表达，包括大鼠颈动脉体（CB）中的白细胞介素（IL）-1受体I和IL-6受体a，CB是一种众所周知的动脉化学感受器，其感测动脉血液中的各种化学刺激。我们证明，IL-1刺激增加CB球细胞的细胞内钙（[Ca 2 +]（i）），释放ATP，并增加颈动脉窦神经的放电率。为了探讨IL-6对CB的作用，我们检测了IL-6对大鼠CB球细胞[Ca ~（2+）]（i）和儿茶酚胺（CA）分泌的影响。钙成像显示，细胞外应用IL-6诱导培养的血管球细胞[Ca 2 +]（i）升高。电流测定法显示局部应用IL-6可诱发血管球细胞CA释放。此外，CA分泌的IL-6的反应被阻断200 μ M镉+，一个众所周知的Ca ~（2+）通道阻滞剂。我们的实验为CB对促炎细胞因子的反应性提供了进一步的证据，并表明CB可能在炎症传感和将这些信息传递到大脑中发挥作用。(C)2009年Wiley-Liss，Inc.
Although abundant evidence indicates mutual regulation between the immune and the central nervous systems, how the immune signals are transmitted to the brain is still an unresolved question. In a previous study we found strong expression of proinflammatory cytokine receptors, including interleukin (IL)-1 receptor I and IL-6 receptor a in the rat carotid body (CB), a well-known arterial chemoreceptor that senses a variety of chemostimuli in the arterial blood. We demonstrated that IL-1 stimulation increases intracellular calcium ([Ca2+](i)) in CB glomus cells, releases ATP, and increases the discharge rate in carotid sinus nerve. To explore the effect of IL-6 on CB, here we examine the effect of IL-6 on [Ca2+](i) and catecholamine (CA) secretion in rat CB glomus cells. Calcium imaging showed that extracellular application of IL-6 induced a rise in [Ca2+](i) in cultured glomus cells. Amperometry showed that local application of IL-6 evoked CA release from glomus cells. Furthermore, the CA secretory response to IL-6 was blocked by 200 mu M Cd2+, a well-known Ca2+ channel blocker. Our experiments provide further evidence for the responsiveness of the CB to proinflammatory cytokines and indicate that the CB might play a role in inflammation sensing and transmission of such information to the brain. (C) 2009 Wiley-Liss, Inc.