V3D enables real-time 3D visualization and quantitative analysis of large-scale biological image data sets.
V3D enables real-time 3D visualization and quantitative analysis of large-scale biological image data sets.
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DOI：
10.1038/nbt.1612
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发表时间：
2010-04
影响因子：
46.9
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V3D 系统在当前的笔记本电脑和台式机上实时提供千兆字节大小的显微镜图像堆栈的三维 (3D) 可视化。结合高度符合人体工程学的功能，可直接在 3D 空间中选择图像的 X、Y、Z 位置以及可视化各种表面对象的叠加，V3D 简化了复杂图像图案的在线分析、测量和校对。 V3D是跨平台的，可以通过插件来增强。我们在 V3D 之上构建了 V3D-Neuron，以从大型大脑图像中重建复杂的 3D 神经元结构。 V3D-Neuron 使我们能够在几分钟内精确数字化果蝇大脑中单个神经元的形态，与其他神经元重建工具相比，可靠性提高了约 17 倍，时间节省了 10 倍。使用 V3D-Neuron，我们证明了在果蝇大脑中构建神经突束 3D 数字图谱的可行性。
The V3D system provides three-dimensional (3D) visualization of gigabyte-sized microscopy image stacks in real time on current laptops and desktops. Combined with highly ergonomic features for selecting an X, Y, Z location of an image directly in 3D space and for visualizing overlays of a variety of surface objects, V3D streamlines the on-line analysis, measurement, and proofreading of complicated image patterns. V3D is cross-platform and can be enhanced by plug-ins. We built V3D-Neuron on top of V3D to reconstruct complex 3D neuronal structures from large brain images. V3D-Neuron enables us to precisely digitize the morphology of a single neuron in a fruit fly brain in minutes, with about 17-fold improvement in reliability and 10-fold savings in time compared to other neuron reconstruction tools. Using V3D-Neuron, we demonstrated the feasibility of building a 3D digital atlas of neurite tracts in the fruit fly brain.