Effects of interleukin-15 (IL-15) on adipose tissue mass in rodent obesity models:: evidence for direct IL-15 action on adipose tissue
Effects of interleukin-15 (IL-15) on adipose tissue mass in rodent obesity models:: evidence for direct IL-15 action on adipose tissue
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DOI：
10.1016/s0304-4165(02)00148-4
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发表时间：
2002-02-15
影响因子：
3
通讯作者：
Quinn, LS
中科院分区：
文献类型：
--
作者：
Alvarez, B;Carbó, N;Quinn, LS
白细胞介素-15 （IL-15）是一种促炎细胞因子，在免疫系统外具有多种功能。先前的研究表明，用IL-15治疗正常大鼠可减少白色脂肪组织（WAT）质量，但尚不清楚这些影响是直接的还是间接的。本研究在瘦素受体阴性的falfa Zucker大鼠和瘦素缺乏的ob/ob小鼠两种肥胖遗传模型中，研究了IL-15对WAT质量和脂质代谢的影响。瘦Zucker大鼠，瘦（+/+）和肥胖小鼠（ob/ob）对IL-15的反应是WAT质量和脂蛋白脂肪酶活性（LPL）的减少，而食物摄入量没有减少。相比之下，falfk Zucker大鼠对IL-15的任何上述脂肪量或脂质代谢测量都没有反应。此外，核糖核酸酶保护实验（RPAs）表明，IL-15受体复合物的三个亚基（γ， β和α）在大鼠和小鼠WAT中均有表达，这表明IL-15对脂肪组织代谢的影响可能是直接的。此外，与瘦Zucker大鼠相比，瘦fa大鼠表达的γ - mac信号受体亚基水平低84%，这表明这种降低可能在脂肪组织对IL-15缺乏反应中发挥作用，并进一步支持IL-15对脂肪组织的直接作用。(C) 2002 Elsevier Science B.V.版权所有
Interleukin-15 (IL-15) is a proinflammatory cytokine with multifunctional effects outside the immune system. Previous studies have indicated that treatment of normal rats with IL-15 reduces white adipose tissue (WAT) mass, but it was unclear if these effects were direct or indirect. In the present study, the effects of IL-15 on WAT mass and lipid metabolism were studied in two genetic models of obesity: the leptin receptor-negative falfa Zucker rat and the leptin-deficient ob/ob mouse. Lean Zucker rats, lean (+/+), and obese mice (ob/ob) responded to IL-15 with reductions in WAT mass and lipoprotein lipase activity (LPL), with no decreases in food intake. In contrast, falfk Zucker rats did not respond to IL-15 administration by any of the above measures of fat mass or lipid metabolism. In addition, ribonuclease protection assays (RPAs) were used to demonstrate that all three subunits (gammac, beta and alpha) of the IL-15 receptor complex are expressed by rat and mouse WAT, suggesting that the effects of IL-15 on adipose tissue metabolism could be direct. Additionally, the fal fa rats expressed 84% lower levels of the gammac signaling receptor subunit than lean Zucker rats, suggesting this decrease may play a role in the lack of adipose tissue response to IL-15 in the falfa genotype and lending further support for a direct action of IL-15 on adipose tissue. (C) 2002 Elsevier Science B.V. All rights reserved.