Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid.
Sterilization of polydimethylsiloxane surface with Chinese herb extract: a new antibiotic mechanism of chlorogenic acid.
复制标题
中药提取物对聚二甲基硅氧烷表面的杀菌：绿原酸的抗菌新机制
DOI：
10.1038/srep10464
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发表时间：
2015-05-21
影响因子：
4.6
通讯作者：
Feng X
中科院分区：
文献类型：
--
作者：
Ren S;Wu M;Guo J;Zhang W;Liu X;Sun L;Holyst R;Hou S;Fang Y;Feng X
用中草药提取物绿原酸（CA）对聚二甲基硅氧烷（PDMS）表面进行涂层处理，解决了目前PDMS表面的灭菌问题。你. coligrows较慢，并具有较高的死亡率上的CA涂覆的PDMS表面。一个更平滑的形态。用原子力显微镜（AFM）观察大肠杆菌细胞壁。与报道的机制不同，CA通过破坏本体溶液中的细胞膜来抑制细菌生长，我们发现CA涂覆的PDMS表面也降低了细胞壁的刚度。的杨氏模量的细胞壁从3至0.8 MPa的减少的报告。出乎意料的是，CA对细菌的群集能力和生物膜稳定性的影响仍然可以观察到，即使在它们已经从CA环境中去除之后，这表明它们对抗生素的抗性在延长的时间内降低。CA涂覆的PDMS表面对三种类型的革兰氏阳性菌和革兰氏阴性菌均显示出比庆大霉素涂覆的PDMS表面更好的抗菌效果。将CA包覆于PDMS表面，不仅解决了PDMS表面的灭菌问题，而且为中草药在科研中的应用提供了新的思路。
Coating of polydimethylsiloxane (PDMS) surface with a traditional Chinese herb extract chlorogenic acid (CA) solves the contemporary problem of sterilization of PDMS surface. TheE. coligrows slower and has a higher death rate on the CA-coated PDMS surfaces. A smoother morphology of theseE. colicell wall is observed by atomic force microscopy (AFM). Unlike the reported mechanism, where CA inhibits bacterial growth by damaging the cell membrane in the bulk solution, we find the CA-coated PDMS surface also decreases the stiffness of the cell wall. A decrease in the Young’s modulus of the cell wall from 3 to 0.8 MPa is reported. Unexpectedly, the CA effect on the swarming ability and the biofilm stability of the bacteria can be still observed, even after they have been removed from the CA environment, indicating a decrease in their resistance to antibiotics for a prolonged time. The CA-coated PDMS surface shows better antibiotic effect against three types of both Gram-positive and Gran-negative bacteria than the gentamicin-coated PDMS surface. Coating of CA on PDMS surface not only solves the problem of sterilization of PDMS surface, but also shines light on the application of Chinese traditional herbs in scientific research.