Nanoparticle-templated assembly of viral protein cages
Nanoparticle-templated assembly of viral protein cages
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DOI：
10.1021/nl0600878
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发表时间：
2006-04-01
期刊：
影响因子：
10.8
通讯作者：
Dragnea, B
中科院分区：
文献类型：
--
作者：
Chen, C;Daniel, MC;Dragnea, B
规则的蛋白质表面在纳米颗粒模板周围的自组装提供了一类新的混合生物材料，其在医学成像和生物分析传感中具有潜在的应用。我们在这里报告的第一个例子，有效的自组装病毒样颗粒（VLP）具有雀麦花叶病毒蛋白外壳和功能化的金核心。本研究表明，功能化的金颗粒可以通过模仿天然病毒的核酸组分的静电行为来启动VLP组装。这些VLP构建体是对称的，其中蛋白质化学计量和包装性质表明与衣壳的二十面体包装相似。此外，观察到VLP的pH诱导的溶胀转变，与天然病毒直接类似。
Self-assembly of regular protein surfaces around nanoparticle templates provides a new class of hybrid biomaterials with potential applications in medical imaging and in bioanalytical sensing. We report here the first example of efficiently self-assembled virus-like particles (VLPs) having a brome mosaic virus protein coat and a functionalized gold core. The present study indicates that functionalized gold particles can initiate VLP assembly by mimicking the electrostatic behavior of the nucleic acid component of the native virus. These VLP constructs are symmetric, with the protein stoichiometry and packaging properties indicating similarity to the icosahedral packing of the capsid. Moreover, a pH-induced swelling transition of the VLPs is observed, in direct analogy to the native virus.