Decreased frequency and function of circulating plasmocytoid dendritic cells (pDC) in hepatitis B virus infected humans
Decreased frequency and function of circulating plasmocytoid dendritic cells (pDC) in hepatitis B virus infected humans
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DOI：
10.1007/s10875-004-6249-y
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发表时间：
2004-11-01
影响因子：
9.1
通讯作者：
Wang, FS
中科院分区：
文献类型：
--
作者：
Duan, XZ;Wang, M;Wang, FS
2型前体浆细胞样树突状细胞(PDC)是抗病毒天然免疫中最重要的细胞类型。为了解PDC在慢性乙型肝炎病毒感染中的作用，采用流式细胞仪分析了25例健康人和116例慢性乙型肝炎患者外周血中PDC的频率和功能，并用ELISA法测定了患者外周血中总PBMCs分泌干扰素-α的水平。慢性乙型病毒性肝炎合并肝硬变(LC)患者外周血中PDC的数量明显减少。PBMC分泌干扰素-α的能力也明显下降。外周血中NK细胞和CD8+T细胞相应减少。我们观察到，在慢性乙肝患者中，拉莫韦丁抗病毒治疗恢复了循环中PDC的数量，PDC频率发生逆转，控制了乙肝病毒的复制，表明这些受试者不太可能完全免疫受损。PDC的降低与LC患者的医院感染有关。我们的结果提示，慢性乙肝患者可能存在循环PDC或NK细胞数量和质量的损害，这可能与乙肝病毒持续感染以及LC患者发生的医院感染有关。
The Type 2 precusor plasmacytoid dendritic cells (pDC) represent the most important cell type in antiviral innate immunity. To understand the function of pDC during hepatitis B virus infection, the frequency and function of circulating pDC were analyzed by flow cytometric analysis, and IFN-alpha secretion of total PBMCs was determined by ELISA assay in 25 healthy subjects and 116 patients at various stages of chronic hepatitis B virus infection (CHB). The number of circulating pDC was found to be significantly lower in patients with CHB and associated liver cirrhosis (LC). The ability of PBMCs to secrete IFN-alpha also decreased significantly. There was a corresponding decrease of circulating NK cells and CD8(+) T cells. We observed that lamuvidine antiviral therapy restored the number of circulating pDC and there was a reversal of pDC frequency with the control of HBV replication in chronic HBV patients, indicating these subjects are unlikely to be totally immunecompromised. The decrease of pDC was found to be related to nosocomial infections in LC patients. Our results suggest that CHB patients probably have a quantitative and qualitative impairment of circulating pDC or NK cells, which may be associated with HBV persistent infection as well as the nosocomial infections that arise in LC patients.