Impaired stimulus-response coupling in association with increased growth rate of HL60 cells.
Impaired stimulus-response coupling in association with increased growth rate of HL60 cells.
复制标题
刺激-反应耦合受损与 HL60 细胞生长速率增加相关。
DOI：
10.1002/jlb.52.2.157
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发表时间：
1992
影响因子：
5.5
通讯作者：
Nielson,CP
中科院分区：
文献类型：
--
作者：
Hadjokas,N;Bayer,C;Nielson,CP
针对HL60细胞在细胞培养的重复传代过程中细胞复制速率的增加，研究了呼吸爆发和刺激-反应耦合的损伤。在45周的培养期间，HL60细胞的复制速度逐渐增加。同时，未分化细胞在三磷酸腺苷诱导的钙动员过程中出现损伤。二甲基亚砜诱导分化的细胞比例逐渐减少。分化细胞在呼吸爆发和β-葡萄糖醛酸酶的分泌方面都出现了损害。此外，在快速增殖的细胞中，cAMP激动剂包括异丙肾上腺素、腺苷和前列腺素E2对呼吸爆发的调节减少。因此，在细胞培养过程中，随着细胞复制速度的增加，刺激-反应耦合发生了多种变化。在使用来自持续维持的细胞群体的HL60细胞的重复样本的研究中，认识到细胞功能的进行性损害可能是重要的。观察到的刺激-反应偶联的损伤可能与肿瘤疾病中未受调控的细胞生长有关。
Impairments in the respiratory burst and stimulus‐response coupling were studied with respect to the increased rate of cell replication that occurred in HL60 cells during repetitive passages in cell culture. During a 45‐week period of culture, HL60 cells developed a progressive increase in rate of replication. Concomitantly, undifferentiated cells developed an impairment in ATP‐induced calcium mobilization. The percentage of cells that could be differentiated with dimethyl sulfoxide progressively diminished. Differentiated cells developed an impairment in both the respiratory burst and secretion of β‐glucuronidase. In addition, regulation of the respiratory burst by cAMP agonists including isoproterenol, adenosine, and prostaglandin E2was reduced in rapidly proliferating cells. Thus, multiple changes in stimulus‐response coupling occur during cell culture in association with an increase in rate of cell replication. It may be important to recognize progressive impairments in cell function in studies using repetitive samples of HL60 cells from a continuously maintained cell population. The observed impairments in stimulus‐response coupling may be relevant to unregulated cell growth in neoplastic disease.