New windows into retroviral RNA structures.
New windows into retroviral RNA structures.
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新窗户进入逆转录病毒RNA结构。
DOI：
10.1186/s12977-018-0393-6
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发表时间：
2018-01-25
期刊：
影响因子：
3.3
通讯作者：
Kenyon JC
中科院分区：
文献类型：
--
作者：
Jayaraman D;Kenyon JC
近年来，病毒和细胞RNA的多重作用变得越来越明显，对它们进行建模的技术变得更加强大，能够更快，更准确地可视化RNA结构。SHAPE（通过引物延伸分析的选择性2'OH酰化）等技术已经彻底改变了该领域，并已用于检查属于许多不同逆转录病毒的RNA。诸如此类的二级结构探测试剂已经通过经由毛细管或下一代测序的更快分析方法的开发而得到帮助，从而允许分析整个基因组和病毒体内的逆转录病毒RNA结构。最近还开发了模拟这些大RNA的三维结构的技术。从这些新的实验技术的结果中可以清楚地看出逆转录病毒RNA在结构和功能上的灵活性。逆转录病毒RNA结构和结构变化控制生命周期的许多阶段，RNA结构本身及其与配体的相互作用都是潜在的新药物靶点。此外，我们对逆转录病毒RNA结构的了解不断增加，这有助于我们了解细胞RNA的形式和功能。
The multiple roles of both viral and cellular RNAs have become increasingly apparent in recent years, and techniques to model them have become significantly more powerful, enabling faster and more accurate visualization of RNA structures. Techniques such as SHAPE (selective 2’OH acylation analysed by primer extension) have revolutionized the field, and have been used to examine RNAs belonging to many and diverse retroviruses. Secondary structure probing reagents such as these have been aided by the development of faster methods of analysis either via capillary or next-generation sequencing, allowing the analysis of entire genomes, and of retroviral RNA structures within virions. Techniques to model the three-dimensional structures of these large RNAs have also recently developed. The flexibility of retroviral RNAs, both structural and functional, is clear from the results of these new experimental techniques. Retroviral RNA structures and structural changes control many stages of the lifecycle, and both the RNA structures themselves and their interactions with ligands are potential new drug targets. In addition, our growing understanding of retroviral RNA structures is aiding our knowledge of cellular RNA form and function.
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影响因子：
30.3
作者：
Kennedy EM;Bogerd HP;Kornepati AV;Kang D;Ghoshal D;Marshall JB;Poling BC;Tsai K;Gokhale NS;Horner SM;Cullen BR
通讯作者：
Cullen BR
影响因子：
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作者：
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通讯作者：
Woehrl, Birgitta M.
影响因子：
64.5
作者：
Fang X;Wang J;O'Carroll IP;Mitchell M;Zuo X;Wang Y;Yu P;Liu Y;Rausch JW;Dyba MA;Kjems J;Schwieters CD;Seifert S;Winans RE;Watts NR;Stahl SJ;Wingfield PT;Byrd RA;Le Grice SF;Rein A;Wang YX
通讯作者：
Wang YX
影响因子：
4.5
作者：
Karabiber, Fethullah;McGinnis, Jennifer L.;Weeks, Kevin M.
通讯作者：
Weeks, Kevin M.
DOI：
10.1073/pnas.1401455111
发表时间：
2014-06-03
影响因子：
11.1
作者：
Bharat, Tanmay A. M.;Menendez, Luis R. Castillo;Briggs, John A. G.
通讯作者：
Briggs, John A. G.