EVIDENCE FOR ADAPTIVE RESPONSE IN A MOLECULAR EPIDEMIOLOGICAL STUDYOF THE INHABITANTS OFA HIGH BACKGROUND-RADIATION AREA OF YANGJIANG, CHINA
EVIDENCE FOR ADAPTIVE RESPONSE IN A MOLECULAR EPIDEMIOLOGICAL STUDYOF THE INHABITANTS OFA HIGH BACKGROUND-RADIATION AREA OF YANGJIANG, CHINA
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中国阳江高本底辐射区居民分子流行病学研究中适应性反应的证据
DOI：
10.1097/hp.0000000000000860
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发表时间：
2018
期刊：
影响因子：
2.2
通讯作者：
温薇
中科院分区：
文献类型：
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作者：
苏世标;周珊宇;温翠菊;邹剑明;张丹英;耿继武;杨敏;刘明;黎丽春;温薇
我们先前的研究表明，中国高本底辐射地区的癌症死亡率低于对照地区，这表明高本底辐射地区可能存在适应性反应。我们的目的是通过一项分子流行病学研究，确定低剂量辐射对广东高本底辐射地区和对照地区人群的DNA氧化损伤水平、DNA损伤修复、抗氧化能力和细胞凋亡的影响，以识别适应性反应。从高本底辐射地区（阳江）和对照地区（恩平）50 - 59岁的男性居民采集血液样本，测定超氧化物歧化酶、谷胱甘肽、过氧化氢酶的活性、总抗氧化能力，以及O6 - 甲基鸟嘌呤 - DNA甲基转移酶基因（MGMT）、人8 - 氧鸟嘌呤DNA N - 糖苷酶1基因（hOGG1）、促凋亡基因和抗凋亡基因、氧化应激相关基因的表达，以及8 - OHdG、TrxR、HSP27和MT - COX2的浓度。高本底辐射地区人群的抗氧化酶活性、DNA修复基因、抗凋亡基因、氧化应激相关基因HSPB1和MT - COX2的相对mRNA表达水平以及抗氧化指标TrxR的浓度与对照人群相比显著增加（p < 0.05）。高本底辐射地区促凋亡基因的相对mRNA表达水平和DNA氧化损伤指标8 - OHdG的浓度与对照地区相比显著降低（p < 0.05）。总之，在长期、自然、高本底电离辐射下，高本底辐射地区居民的DNA损伤修复能力和抗氧化能力可能增强。此外，它可能诱导细胞存活基因表达上调和凋亡基因表达下调。可以推测，增强的抗氧化和DNA修复能力以及对细胞凋亡的抑制可能在高本底辐射地区低剂量辐射的适应性反应中起重要作用。
Our previous studies have shown that cancer mortality in high background-radiation areas of China was lower than that in a control area, indicating the possibility of an adaptive response in high background-radiation areas. Our aim is to determine the effect of low-dose radiation on the level of DNA oxidative damage, DNA damage repair, antioxidant capacity, and apoptosis in high background-radiation area and control area populations of Guangdong through a molecular epidemiological study in order to identify adaptive response. Blood samples were collected from male residents aged 50 to 59 y in a high background-radiation area (Yangjiang) and a control area (Enping), and activities of superoxide dismutase, glutathione, catalase, total antioxidant capacity,and expression of O6‐methylguanine-DNA methyltransferase gene (MGMT), human 8‐oxoguanine DNA N-glycosylase 1 gene (hOGG1), proapoptotic genes and antiapoptotic genes, oxidative-stress-related genes, aswell as concentrations of 8‐OHdG, TrxR, HSP27, and MT-COX2 were determined. The activities of antioxidative enzymes, relative mRNA expression level of DNA repair genes, antiapoptotic genes, oxidative-stress-related genes HSPB1 and MT-COX2, and the concentration of antioxidant index TrxR in the high background-radiation area population increased.significantly compared to the control population (p < 0.05). The relative mRNA expression level of proapoptotic genes and the concentration of DNA oxidative damage index 8‐OHdG were significantly lower in the high background-radiation area compared to those in the control area (p < 0.05). In conclusion, under longterm,.natural, high background, ionizing radiation, DNA damagerepair capacity and antioxidant capacity of inhabitants in the high background-radiation area may be enhanced. Additionally, it could induce up regulation of cell-survival gene expression and down regulation of apoptotic gene expression. It might be speculated that enhanced antioxidant and DNA repair capacity and inhibition of apoptosis might play important roles in adaptive response of low-dose radiation in high background-radiation areas.