Comparative study of normal and osteoarthritic canine synovial fluid using 500 MHz 1H magnetic resonance spectroscopy
Comparative study of normal and osteoarthritic canine synovial fluid using 500 MHz 1H magnetic resonance spectroscopy
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DOI：
10.1002/jor.1100170211
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发表时间：
1999-03-01
影响因子：
2.8
通讯作者：
Marshall, KW
中科院分区：
文献类型：
--
作者：
Damyanovich, AZ;Staples, JR;Marshall, KW
高分辨率H-1核磁共振波谱已被用于研究和比较正常和骨关节炎滑液在犬骨关节炎模型的代谢谱。骨关节炎滑液的光谱显示，与健康正常犬滑液相比，（a）乳酸盐、丙酮酸盐、脂蛋白相关脂肪酸和甘油以及酮羟基丁酸盐和羟基异丁酸盐的浓度升高，（B）葡萄糖水平降低，（c）N-乙酰糖蛋白、乙酸盐和乙酰胺水平升高。还观察到氨基酸丙氨酸和异亮氨酸的浓度增加。这些结果表明：（a）犬骨关节炎的关节内环境比正常关节更缺氧和酸中毒，（B）脂解作为骨关节炎的能量来源可能发挥越来越重要的作用，和（c）滑液的N-乙酰糖蛋白聚合物组分（主要是透明质酸）似乎通过乙酰胺中间体越来越多地碎裂并降解成乙酸盐，伴有进行性骨关节炎。观察到的犬骨关节炎滑液的生化特征的变化可能有助于了解关节炎疾病引起的关节代谢的改变，并有助于识别骨关节炎的潜在标志物。
High resolution H-1 nuclear magnetic resonance spectroscopy has been used to investigate and compare the metabolic profiles of normal and osteoarthritic synovial fluids in a canine model of osteoarthritis. The spectra of osteoarthritic synovial fluid showed (a) increased concentrations of lactate, pyruvate, lipoprotein-associated fatty acids, and glycerol as well as the ketones hydroxybutyrate and hydroxyisobutyrate, (b) reduced levels of glucose, and (c) elevated levels of N-acetylglycoproteins, acetate, and acetamide compared with healthy normal canine synovial fluid. An increase was also observed in the concentrations of the amino acids alanine and isoleucine. These results suggest that (a) the intraarticular environment in canine osteoarthritis is more hypoxic and acidotic than in a normal joint, (b) lipolysis may play an increasingly important role as a source of energy in osteoarthritis, and (c) the N-acetylglycoprotein polymer component of synovial fluid (mostly hyaluronan) seems to be increasingly fragmented and degraded into acetate by way of an acetamide intermediate with progressive osteoarthritis. The observed changes in the biochemical profile of canine osteoarthritic synovial fluid may be useful in understanding alterations in joint metabolism consequent to arthritic diseases and helpful in identifying potential markers of osteoarthritis.