Generating elastic, biodegradable polyurethane/poly(lactide-co-glycolide) fibrous sheets with controlled antibiotic release via two-stream electrospinning
Generating elastic, biodegradable polyurethane/poly(lactide-co-glycolide) fibrous sheets with controlled antibiotic release via two-stream electrospinning
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DOI：
10.1021/bm701201w
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发表时间：
2008-04-01
影响因子：
6.2
通讯作者：
Wagner, William R.
中科院分区：
文献类型：
--
作者：
Hong, Yi;Fujimoto, Kazuro;Wagner, William R.
损伤控制剖腹手术通常用于预防创伤后的骨筋膜室综合征，但会带来组织的新风险，包括感染。为了满足生物材料改善腹部剖腹手术管理的需求，我们制造了一种弹性纤维复合片，具有两种不同的亚微米纤维群：可生物降解的聚（酯氨基甲酸酯）尿素（PEUU）和聚（丙交酯-乙交酯）（PLGA），其中PLGA负载有抗生素四环素盐酸盐（PLGA-tet）。开发了双流静电纺丝装置，以产生 PEUU 和 PLGA-tet 纤维的均匀混合物。复合片材柔韧，断裂应变超过 200%，拉伸强度为 5-7 MPa，缝合线保持能力高。 PEUU 纤维的共混显着降低了 PLGA-tet 片材在缓冲液中观察到的收缩率，从 50% 降至 15%，同时赋予复合材料弹性性能。在 37 摄氏度的缓冲液中孵育 7 天后，复合片材仍保持抗菌活性。体内研究表明，植入材料可以防止受污染的大鼠腹壁模型中脓肿的形成。这些结果证明了双流静电纺丝方法的优点，其中机械和控释性能由独立的纤维群体贡献，并且该复合材料对腹壁闭合的适用性。
Damage control laparotomy is commonly applied to prevent compartment syndrome following trauma but is associated with new risks to the tissue, including infection. To address the need for biomaterials to improve abdominal laparotomy management, we fabricated an elastic, fibrous composite sheet with two distinct submicrometer fiber populations: biodegradable poly(ester urethane) urea (PEUU) and poly(lactide-co-glycolide) (PLGA), where the PLGA was loaded with the antibiotic tetracycline hydrochloride (PLGA-tet). A two-stream electrospinning setup was developed to create a uniform blend of PEUU and PLGA-tet fibers. Composite sheets were flexible with breaking strains exceeding 200%, tensile strengths of 5-7 MPa, and high suture retention capacity. The blending of PEUU fibers markedly reduced the shrinkage ratio observed for PLGA-tet sheets in buffer from 50% to 15%, while imparting elastomeric properties to the composites. Antibacterial activity was maintained for composite sheets following incubation in buffer for 7 days at 37 degrees C. In vivo studies demonstrated prevention of abscess formation in a contaminated rat abdominal wall model with the implanted material. These results demonstrate the benefits derivable from a two-stream electrospinning approach wherein mechanical and controlled-release properties are contributed by independent fiber populations and the applicability of this composite material to abdominal wall closure.