Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.
Phosphorus-31 nuclear magnetic resonance spectroscopy of toad retina.
复制标题
蟾蜍视网膜的磷 31 核磁共振波谱。
DOI：
10.1016/s0006-3495(89)82691-8
复制
发表时间：
1989
影响因子：
3.4
通讯作者：
Ebrey,TG
中科院分区：
文献类型：
--
作者：
Apte,DV;Koutalos,Y;McFarlane,DK;Dawson,MJ;Ebrey,TG
Phosphorus-31 nuclear magnetic resonance (31P-NMR) spectra were obtained from living toad retinae and toad retinal extracts at 4 degrees C. Several phosphorus metabolites--nucleoside di- and triphosphates (NTP), phosphocreatine, phosphodiesters, inorganic phosphate, and phosphomonoesters--were identified from the spectra of whole retinae. The intracellular pH was determined to be 7.27 +/- 0.06 at 4 degrees C and the intracellular MgNTP/NTP ratio was at least 0.77. These results are consistent with those reported by other techniques, and they show that 31P-NMR spectroscopy can be used for noninvasively and quantitatively studying the metabolism of living toad retinae, and for monitoring its changes over time.
