Optical sensing device for online calcium detection in extracorporeal blood purification
Optical sensing device for online calcium detection in extracorporeal blood purification
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DOI：
10.1016/j.snb.2014.12.055
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发表时间：
2015-03
影响因子：
8.4
通讯作者：
E. John;C. Cheng;A. Bornschlegel;O. Brüggemann;T. Posnicek;M. Brandl
中科院分区：
文献类型：
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作者：
E. John;C. Cheng;A. Bornschlegel;O. Brüggemann;T. Posnicek;M. Brandl
研制了一种基于水凝胶固定荧光染料的钙敏感荧光传感器，该荧光染料被放置在透明测量细胞的内壁上。离子化的钙（iCa）敏感荧光团俄勒冈-绿bapta-1葡聚糖（og -葡聚糖）与钙离子反应，导致荧光强度与iCa浓度成正比增加。为了测量ica灵敏荧光传感器的光信号，研制了一种合适的高灵敏度光学测量装置。该传感器的目的是测量体外血液循环中柠檬酸钙抗凝过程中的iCa浓度。因此，新开发的钙传感器可以为治疗过程中钙离子的准确在线测量和治疗后钙离子的调整提供增强。
A calcium-sensitive fluorescence sensor based on a fluorescent dye immobilized in a hydrogel that is placed onto the inner wall of a transparent measuring cell was developed. The ionized calcium (iCa)-sensitive fluorophore Oregon-Green bapta-1 dextran (OG-dextran) reacts with calcium ions, resulting in an increase in the fluorescence intensity that is proportional to the concentration of iCa. To measure the optical signal of the iCa-sensitive fluorescence sensor, an appropriate and very sensitive optical measuring device was developed. The aim of this sensor is to measure the iCa concentration during extracorporeal citrate–calcium anticoagulation in the extracorporeal blood circuit. Thus, the newly developed calcium sensor can offer an enhancement for the accurate online measurement of iCa during treatment and the adjustment of iCa afterwards.