Comparison of subcellular partitioning, distribution, and internal speciation of Cu between Cu-tolerant and naïve populations of Dendrodrilus rubidus Savigny.
Comparison of subcellular partitioning, distribution, and internal speciation of Cu between Cu-tolerant and naïve populations of Dendrodrilus rubidus Savigny.
复制标题
铜耐铜种群和红树突蝠幼稚种群之间铜的亚细胞分配、分布和内部形态的比较。
DOI：
10.1021/es800172g
复制
发表时间：
2008
影响因子：
11.4
通讯作者：
Willie J.G.M. Peijnenburg
中科院分区：
文献类型：
--
作者：
Becky E. Arnold;Mark E. Hodson;John Charnock;Willie J.G.M. Peijnenburg
在考虑污染场地生态和生态风险评估时，一个关键问题是看起来不受污染物积累影响的生物体对这些污染物是耐受还是耐受。来自Coniston铜矿的一群Rendrodrilus rubidus Savigny蚯蚓，与附近的非暴露于铜的种群相比，对铜的耐受性和积累都有所增加。在铜修饰土壤中暴露于250mgCukg(-1)14d后，测定了两个种群不同身体部位(后部、前部、体壁)的总铜的分配。铜在Coniston蚯蚓体内的含量较高，但不同种群间不同部位铜的相对比例相同。用扩展X射线吸收精细结构谱(EXAFS)测定了铜的形态。暴露土壤中铜与O原子配位，而蚯蚓中铜与S原子配位。不同种群的蚯蚓在物种组成上没有差异。在另一项实验中，将蚯蚓暴露于一定范围的铜浓度(200-700毫克铜公斤(-1))。测定了累积铜的亚细胞分配。Coniston蚯蚓积累了更多的铜，但不同种群之间不同组分(胞浆和GT；颗粒状和GT；组织碎片、细胞膜和完整细胞)中铜的相对比例是相同的。结果表明，通过扩大存在于附近非铜暴露人群中的相同的铜储存库，康氏金线虫能够在富铜的康尼斯顿铜矿土壤中生存。
When considering contaminated site ecology and ecological risk assessment a key question is whether organisms that appear unaffected by accumulation of contaminants are tolerant or resistant to those contaminants. A population of Dendrodrilus rubidus Savigny earthworms from the Coniston Copper Mines, an area of former Cu mining, exhibit increased tolerance and accumulation of Cu relative to a nearby non-Cu exposed population. Distribution of total Cu between different body parts (posterior, anterior, body wall) of the two populations was determined after a 14 day exposure to 250 mg Cu kg(-1) in Cu-amended soil. Cu concentrations were greater in Coniston earthworms but relative proportions of Cu in different body parts were the same between populations. Cu speciation was determined using extended X-ray absorption fine structure spectroscopy (EXAFS). Cu was coordinated to O atoms in the exposure soil but to S atoms in the earthworms. There was no difference in this speciation between the different earthworm populations. In another experiment earthworms were exposed to a range of Cu concentrations (200-700 mg Cu kg(-1)). Subcellular partitioning of accumulated Cu was determined. Coniston earthworms accumulated more Cu but relative proportions of Cu in the different fractions (cytosol > granular > tissue fragments, cell membranes, and intact cells) were the same between populations. Results suggest that Coniston D. rubidus are able to survive in the Cu-rich Coniston Copper Mines soil through enlargement of the same Cu storage reservoirs that exist in a nearby non-Cu exposed population.