Effects of rosightazone and high fat diet on lipase/esterase expression in adipose tissue
Effects of rosightazone and high fat diet on lipase/esterase expression in adipose tissue
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DOI：
10.1016/j.bbalip.2006.11.009
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发表时间：
2007-02-01
影响因子：
4.8
通讯作者：
Kraemer, Fredric B.
中科院分区：
文献类型：
--
作者：
Shen, Wen-Jun;Patel, Shailja;Kraemer, Fredric B.
已经通过活性的功能测定或通过蛋白质组学分析报道了脂肪组织中的许多细胞内脂肪酶/酯酶。在目前的工作中，我们已经研究了脂肪酶/酯酶家族的12个成员的相对表达水平，发现在白色脂肪组织。我们发现ATGL和HSL的相对mRNA水平是最丰富的，是TGH或ADPN的2-3倍;而其他细胞内中性脂肪酶/酯酶的表达水平明显较低。高脂喂养并没有改变大多数脂肪酶/酯酶的mRNA表达水平，但降低CGI-58和WBSCR 21。同样，罗格列酮处理没有改变大多数脂肪酶/酯酶的mRNA表达水平，但增加了ATGL、TGH、CGI-58和WBSCR 21，同时降低了ADPN。来自HSL-/-小鼠的WAT未显示任何脂肪酶/酯酶的代偿性增加，而是大多数脂肪酶/酯酶的mRNA水平降低。相反，来自HSL-/-小鼠的BAT显示ATGL表达增加，以及ES-1、APEH和WBSCR 21的减少。一些脂肪酶的免疫反应蛋白水平的分析证实了mRNA的结果。总之，这些数据突出了参与脂解的细胞内中性脂肪酶/酯酶的表达调节的复杂性。(c)2006 Elsevier B. V.保留所有权利。
A number of intracellular lipase/esterase have been reported in adipose tissue either by functional assays of activity or through proteomic analysis. In the current work, we have studied the relative expression level of 12 members of the lipase/esterase family that are found in white adipose tissue. We found that the relative mRNA levels of ATGL and HSL are the most abundant, being 2-3 fold greater than TGH or ADPN; whereas other intracellular neutral lipase/esterases were expressed at substantially lower levels. High fat feeding did not alter the mRNA expression levels of most lipase/esterases, but did reduce CGI-58 and WBSCR21. Likewise, rosiglitazone treatment did not alter the mRNA expression levels of most lipase/esterases, but did increase ATGL, TGH, CGI-58 and WBSCR21, while reducing ADPN. WAT from HSL-/- mice showed no compensatory increase in any lipase/esterases, rather mRNA levels of most lipase/esterases were reduced. In contrast, BAT from HSL-/- mice showed an increase in ATGL expression, as well as a decrease in ES-1, APEH and WBSCR21. Analysis of the immunoreactive protein levels of some of the lipases confirmed the results seen with mRNA. In conclusion, these data highlight the complexity of the regulation of the expression of intracellular neutral lipase/esterases involved in lipolysis. (c) 2006 Elsevier B.V. All rights reserved.