Monitoring Neural Activities in the VTA in Response to Nicotine Intake Using a Novel Implantable Microimaging Device
Monitoring Neural Activities in the VTA in Response to Nicotine Intake Using a Novel Implantable Microimaging Device
复制标题
DOI：
10.1109/access.2020.2985705
复制
发表时间：
2020-01-01
期刊：
影响因子：
3.9
通讯作者：
Akay, Metin
中科院分区：
文献类型：
--
作者：
Sunaga, Yoshinori;Ohta, Yasumi;Akay, Metin
测量啮齿动物的脑深部区域一直是研究脑深部活动的最重要方法之一，部分原因是该区域的访问有限。为了解决这个问题，我们设计并制造了一种植入式显微成像装置，用于啮齿动物脑深部的荧光成像。在这项研究中，我们建立了一种新的制造方法的平面和均匀的荧光过滤器的植入式显微成像设备，获得更高质量的图像，并旨在显示该设备在大鼠腹侧被盖区（VTA）的性能。我们通过腺相关病毒（AAV）注射将GCaMP 6引入大鼠的VTA。荧光强度的变化与尼古丁给药相关，并通过使用我们定制的植入式显微成像装置进行观察。我们发现，尼古丁给药显著影响了腹侧被盖区不同亚区的DA神经元的兴奋，在尼古丁暴露后6-10分钟之间达到最大激活，然后在31-35分钟后降低。因此，我们相信我们的植入式显微成像设备可以通过使用GCaMP来监测腹侧被盖区子区域内响应急性尼古丁暴露的神经激活。
Measurements of the deep brain area of rodents has been one of the most important methods to study deep brain activities, in part due to limited access of this region. In order to address this problem, we have designed and fabricated an implantable microimaging device for fluorescence imaging in the deep brain of rodents. In this study, we established a new fabrication method of a flat and uniformed fluorescence filter for an implantable microimaging device which obtained higher quality images, and aimed to show the performance of the device in the ventral tegmental area (VTA) of rats. We introduced GCaMP6s in the VTA of rats by adeno-associated viral (AAV) injection. The change in the fluorescence intensity was associated with nicotine administration and was observed by using our custom implantable microimaging device. We showed that nicotine administration significantly affected the excitation of DA neurons in different sub-regions of the VTA, reaching a maximum activation between 6-10 minutes following nicotine exposure, followed by a decrease after 31-35 minutes. Therefore, we believe our implantable microimaging device can potentially be used to monitor the neural activation within the sub-regions of the VTA in response to acute nicotine exposure by using GCaMP.