Integration of the blaNDM-1 carbapenemase gene into a novel SXT/R391 integrative and conjugative element in Proteus vulgaris
Integration of the blaNDM-1 carbapenemase gene into a novel SXT/R391 integrative and conjugative element in Proteus vulgaris
复制标题
将 blaNDM-1 碳青霉烯酶基因整合到普通变形杆菌中的新型 SXT/R391 整合和接合元件中
DOI：
10.1093/jac/dkaa068
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发表时间：
2020-06-01
影响因子：
5.2
通讯作者：
Wang, Hong-Ning
中科院分区：
文献类型：
--
作者：
Kong, Ling-Han;Xiang, Rong;Wang, Hong-Ning
目的：方法：从2018年中国四川省1头腹泻病猪的粪便拭子中分离到碳青霉烯类耐药的普通变形杆菌BC 22株。通过PCR筛选碳青霉烯酶基因的存在。结果：普通毕赤酵母BC 22株携带碳青霉烯酶基因bla（NDM-1）。WGS数据显示bla（NDM-1）位于截短的ISAba 125复合转座子中。碳青霉烯类耐药基因bla（NDM-1）和其他20个耐药基因，包括多重耐药基因cfr和双功能氨基糖苷类/喹诺酮类耐药基因aac（6 '）-lb-cr，被定位在一个新的SXT/R391整合接合元件（ICE）中。结论：将碳青霉烯酶基因blaNDM-1整合到SXT/R391 ICE中，获得了完整的碳青霉烯酶基因blaNDM-1。我们的研究强调，这种SXT/R391 ICE可能促进临床上重要的耐药基因如bla（NDM-1）、cfr和aac（6 '）-lb-cr的传播。
Objectives: To characterize the genetic environment of the carbapenem resistance determinant in Proteus vulgaris of swine origin.Methods: The carbapenem-resistant P. vulgaris strain BC22 was isolated from a faecal swab from a diseased pig with diarrhoea in Sichuan Province of China in 2018. The presence of carbapenemase genes was screened by PCR. WGS and bioinformatics analysis were performed to analyse the genetic environment of the carbapenem resistance determinant.Results: P. vulgaris strain BC22 was found to harbour the carbapenemase gene bla(NDM-1). WGS data revealed that bla(NDM-1) was located in a truncated ISAba125 composite transposon. The carbapenem resistance gene bla(NDM-1) and 20 other resistance genes, including the multiresistance gene cfr and the bifunctional aminoglycoside/quinolone resistance gene aac(6')-lb-cr, were located in a novel SXT/R391 integrative and conjugative element (ICE). This new SXT/R391 ICE of 148.7 kb was chromosomally located, and could be transferred to Escherichia coli.Conclusions: Here, we report a carbapenemase gene, blaNDM-1, integrated into an SXT/R391 ICE. Our study highlights that this SXT/R391 ICE may facilitate the dissemination of clinically important resistance genes such as bla(NDM-1), cfr and aac(6')-lb-cr.