Lipids Are the Preferred Substrate of the Protist Naegleria gruberi, Relative of a Human Brain Pathogen.
Lipids Are the Preferred Substrate of the Protist Naegleria gruberi, Relative of a Human Brain Pathogen.
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DOI：
10.1016/j.celrep.2018.09.055
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发表时间：
2018-10-16
期刊：
影响因子：
8.8
通讯作者：
Tielens AGM
中科院分区：
文献类型：
--
作者：
Bexkens ML;Zimorski V;Sarink MJ;Wienk H;Brouwers JF;De Jonckheere JF;Martin WF;Opperdoes FR;van Hellemond JJ;Tielens AGM
格鲁伯耐格里阿米巴是一种自由生活的非致病性阿米巴鞭毛虫，是福氏耐格里阿米巴的近亲，福氏耐格里阿米巴是一种引起原发性阿米巴脑膜脑炎（PAM）的致命病原体。存在对格鲁伯猪笼草的基因组分析，但缺乏其核心能量代谢或体内生长底物的生理学证据。在这里，我们表明，N. gruberi滋养体需要氧气的正常运作和生长，他们回避葡萄糖和氨基酸作为生长底物。滋养体的生长主要依赖于脂质氧化通过线粒体呼吸链的分支，其两端需要氧作为最终的电子受体。因此，生长中的格鲁伯纳氏线虫滋养体具有严格的有氧能量代谢，对脂肪酸的氧化具有显著的底物偏好。对福氏猪笼草基因组数据的分析以及与格鲁伯猪笼草基因组数据的比较表明，福氏猪笼草具有相同的代谢类型。专门化氧依赖性脂质分解代表了原生生物的另一种代谢策略。格鲁贝耐格里阿米巴是一种严格的需氧生物，需要氧气来维持其正常的功能和生长。格鲁贝耐格里阿米巴是原生生物中的一种独特的生物，它喜欢将脂质而不是葡萄糖作为能量来源。脂质分解通过一个分支的呼吸链进行，两端都使用氧气。表明格鲁伯纳氏变形虫更喜欢以脂类为生，因为它们需要氧气，这种生活方式在原生生物中基本上是未知的。这挑战了现有关于其能量代谢的观点，并对其致病性亲属福氏纳氏菌（N.fowleri）的治疗产生了影响，福氏纳氏纳氏菌是原发性阿米巴脑膜脑炎（PAM）的食脑剂。
Naegleria gruberi is a free-living non-pathogenic amoeboflagellate and relative of Naegleria fowleri, a deadly pathogen causing primary amoebic meningoencephalitis (PAM). A genomic analysis of N. gruberi exists, but physiological evidence for its core energy metabolism or in vivo growth substrates is lacking. Here, we show that N. gruberi trophozoites need oxygen for normal functioning and growth and that they shun both glucose and amino acids as growth substrates. Trophozoite growth depends mainly upon lipid oxidation via a mitochondrial branched respiratory chain, both ends of which require oxygen as final electron acceptor. Growing N. gruberi trophozoites thus have a strictly aerobic energy metabolism with a marked substrate preference for the oxidation of fatty acids. Analyses of N. fowleri genome data and comparison with those of N. gruberi indicate that N. fowleri has the same type of metabolism. Specialization to oxygen-dependent lipid breakdown represents an additional metabolic strategy in protists. Naegleria gruberi is a strict aerobe and needs oxygen for normal functioning and growth Unique among protists, N. gruberi prefers lipids over glucose as an energy source Lipid breakdown proceeds via a branched respiratory chain, both ends using oxygen N. fowleri, the fatal human brain amoeba, is predicted to have the same food preference Bexkens et al. show that N. gruberi amoebae live preferably on lipids, for which they need oxygen, a lifestyle largely unknown among protists. This challenges existing views about its energy metabolism, with implications for treatment of its pathogenic relative, N. fowleri, the brain-eating agent of primary amoebic meningoencephalitis (PAM).
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