Pharmacological evaluation for improvement of Kanazawa Sutra, medicinal thread for anal fistula
Pharmacological evaluation for improvement of Kanazawa Sutra, medicinal thread for anal fistula
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改进金泽经、肛瘘药线的药理评价
DOI：
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发表时间：
2016
影响因子：
3.3
通讯作者：
M. Mikage
中科院分区：
文献类型：
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作者：
T. Yokogawa;Yohei Sasaki;Hirokazu Ando;Katsuya Yamamoto;M. Mikage
金泽经（KanS）是一种用于治疗肛瘘的药用线。它被用作印度草药中使用的Kshara Sutra（KS）的替代品。坎S由无花果胶乳（Ficus carica latex，FCL）、辣椒酊（Capsicum annuum tincture，CAT）、牛膝（牛膝为全草灰）和姜黄粉（Curcuma longa rhizoma，RUM）组成。在这项研究中，我们评估KanS的成分，通过测量一氧化氮（NO）的产生在小鼠巨噬细胞样细胞系J774.1，以及检查细胞毒性大鼠骨骼肌成肌细胞（L6）和L6分化，以提高其药理作用。我们重点研究了野桐树皮（Mallotus grandbark，MJB），它在日本药典中有描述，属于大戟科。以与KanS成分评价相似的方式评价其生物活性。我们发现，MJB提取物显示出类似的生物活性的大戟属neriifolia胶乳（ENL），KS的成分。我们认为KanS的NO抑制活性主要是通过抑制L6细胞的增殖来实现的，而其对L6细胞的细胞毒性和对L6细胞分化的抑制活性主要是通过抑制L6细胞增殖和抑制FCL来实现的。由于CAT无特征活性，用MJB替代CAT可提高KanS的生物活性和治疗肛瘘的能力。
Kanazawa Sutra (KanS) is a medicinal thread that is used for the treatment of anal fistula. It is used as a substitute for Kshara Sutra (KS) which is used in Ayurvedic medicine. KanS is composed of Ficus carica latex (FCL), Capsicum annuum tincture (CAT), Achyranthes fauriei Kshara (which is processed ash from the whole plant) and powdered Curcuma longa rhizome (CLR). In this study, we evaluated the ingredients of KanS by measuring nitric oxide (NO) production in murine macrophage-like cell line J774.1 as well as examining cytotoxicity to rat skeletal muscle myoblasts (L6) and L6 differentiation, with a view to improving its pharmacological effect. We focused on Mallotus japonicus bark (MJB), which is described in the Japanese Pharmacopeia and belongs to the Euphorbiaceae family. Its biological activities were evaluated in a similar manner to the evaluation of KanS ingredients. We found that MJB extracts showed similar biological activity to Euphorbia neriifolia latex (ENL), an ingredient of KS. We conclude that the NO inhibitory activity of KanS is mainly due to CLR, and its cytotoxicity to L6 and inhibitory activity on L6 differentiation are mainly due to CLR and FCL. As CAT has no characteristic activity, the biological activity and the anal fistula treatment ability of KanS would be improved by substituting MJB for CAT.
影响因子：
6.1
作者：
Siu PM;Wang Y;Alway SE
通讯作者：
Alway SE