Complete nucleotide sequence of dog heart creatine kinase mRNA: conservation of amino acid sequence within and among species.
Complete nucleotide sequence of dog heart creatine kinase mRNA: conservation of amino acid sequence within and among species.
复制标题
狗心脏肌酸激酶 mRNA 的完整核苷酸序列：物种内和物种间氨基酸序列的保守性。
DOI：
10.1073/pnas.82.24.8394
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Strauss,A
中科院分区：
文献类型：
--
作者：
Roman,D;Billadello,J;Gordon,J;Grace,A;Sobel,B;Strauss,A
肌酸激酶（CK; EC 2.7.3.2）在脑和肌肉的能量代谢中起重要作用。CK同工酶的表达在发育过程中受到调节，并且具有组织特异性。为了明确犬CK同工酶的结构和阐明其表达的调控机制，从犬心肌中分离CK cDNA克隆。心肌CK mRNA编码381个氨基酸。mRNA的非翻译区在5'端包含至少38个碱基，在poly（A）尾之前包含正好345个碱基。测定了狗肌CK亚基和脑CK亚基的部分蛋白质序列，并与心肌酶和其它物种的肌CK亚基的氨基酸序列进行了比较。来自不同物种的M CK亚基共享非常高程度（83-96%）的序列同一性。狗的M和B亚基具有广泛的序列同一性（74%），这种相似程度以前没有被怀疑过。Southern杂交分析表明，该家系存在一个CK基因家族。这些观察结果意味着，在M CK亚基结构的进化变化的限制，需要保存的功能特性以外的激酶活性。这种保守性与M亚基在心肌和骨骼肌中起结构作用的可能性一致。
Creatine kinase (CK; EC 2.7.3.2) plays an important role in energy metabolism in brain and muscle. Expression of CK isoenzymes is regulated during development and is tissue specific. To define the structures of canine CK isoenzymes and to elucidate the mechanism of regulation in their expression, CK cDNA clones from dog myocardium were isolated. Myocardial CK mRNA is predicted to encode a protein of 381 amino acids. The nontranslated regions of the mRNA comprise at least 38 bases at the 5' end and exactly 345 bases before the poly(A) tail. Partial protein sequences of dog muscle (M) CK and brain (B) CK subunits were determined and compared with the derived amino acid sequence of the myocardial enzyme and of M CK subunits of other species. The M CK subunits from different species share a very high degree (83-96%) of sequence identity. Dog M and B subunits share extensive sequence identity (74%), a degree of similarity not previously suspected. Southern blot analysis suggests that a CK gene family exists. These observations imply that evolutionary changes in the M CK subunit structure are constrained by the need for preservation of functional properties other than the kinase activity. This conservation is consistent with the possibility that the M subunit plays a structural role in cardiac and skeletal muscle.