Heparin-agarose chromatography for the purification of tetrahydrofolate utilizing enzymes: C1-tetrahydrofolate synthase and 10-formyltetrahydrofolate synthetase.
Heparin-agarose chromatography for the purification of tetrahydrofolate utilizing enzymes: C1-tetrahydrofolate synthase and 10-formyltetrahydrofolate synthetase.
复制标题
肝素-琼脂糖色谱法利用酶纯化四氢叶酸：C1-四氢叶酸合成酶和10-甲酰四氢叶酸合成酶。
DOI：
10.1016/0003-2697(87)90035-2
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发表时间：
1987
影响因子：
2.9
通讯作者：
J. Rabinowitz
中科院分区：
文献类型：
--
作者：
C. Staben;T. Whitehead;J. Rabinowitz
Rapid and convenient purification procedures based upon heparin-agarose chromatography for C1-tetrahydrofolate synthase from Saccharomyces cerevisiae and 10-formyltetrahydrofolate synthetase from Clostridium acidi-urici have been developed. The purification of the yeast enzyme involves three chromatographic steps that can be done rapidly, with no intervening dialyses, and results in high yield. The first step alone, heparin-agarose chromatography, is sufficient to purify the enzyme from yeast bearing a cloned copy of the ADE3 gene that overexpresses the protein. The other steps in the purification from wild-type yeast are matrex gel red A and phenyl-Sepharose chromatography. The purification of the clostridial enzyme involves protamine sulfate fractionation and heparin-agarose chromatography. Heparin-agarose also binds two other enzymes that use tetrahydrofolate, 5,10-methenyltetrahydrofolate cyclohydrolase and 5,10-methylenetetrahydrofolate dehydrogenase. Thus, heparin-agarose should prove useful in purification of a variety of enzymes that utilize tetrahydrofolate or its derivatives as a cofactor.
甲酸乙酸梭菌 5,10-次甲基四氢叶酸环化水解酶的纯化和性质。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Clark,JE;Ljungdahl,LG
通讯作者：
Ljungdahl,LG
伍氏醋杆菌 NAD 依赖性 5,10-亚甲基四氢叶酸脱氢酶的纯化和特性。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ragsdale,SW;Ljungdahl,LG
通讯作者：
Ljungdahl,LG