Effect of penicillin G on Staphylococcus aureus phagocytosed by human monocytes.
Effect of penicillin G on Staphylococcus aureus phagocytosed by human monocytes.
复制标题
青霉素G对人单核细胞吞噬金黄色葡萄球菌的影响。
DOI：
10.1093/infdis/153.3.586
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
R. van Furth
中科院分区：
文献类型：
--
作者：
P. J. van den Broek;L. Buys;H. Mattie;R. van Furth
将青霉素G对人单核细胞吞噬后的金黄色葡萄球菌的作用与对未被吞噬的细菌的作用进行比较。通过将单核细胞和预调理的金黄色葡萄球菌在37°C下孵育3分钟获得胞内金黄色葡萄球菌；通过在碎冰中冷却试管来停止吞噬作用。通过差速离心去除非吞噬细菌，然后洗涤细胞两次。溶葡萄球菌素的形态学研究证实，在此过程后大约 70% 的细菌定位于细胞内。青霉素 G 的抗菌活性通过将含有摄入的金黄色葡萄球菌的单核细胞与药物在 37°C 下孵育来评估，并以不同的时间间隔对活细胞相关细菌的数量进行微生物学评估。通过在培养过程中省略血清来灭活单核细胞的细胞内杀伤机制，从而可以测量除了细胞的杀菌活性之外的青霉素G的效果。将预调理的金黄色葡萄球菌在含有药物的培养基中孵育后，评估青霉素 G 对非吞噬细菌的作用。结果表明，青霉素G对细胞内金黄色葡萄球菌的作用比对非吞噬金黄色葡萄球菌的作用强2至7倍。
The effect of penicillin G on Staphylococcus aureus after phagocytosis by human monocytes was compared with the effect on nonphagocytosed bacteria. Intracellular S. aureus was obtained by incubation of monocytes and preopsonized S. aureus for 3 min at 37 C; phagocytosis was stopped by cooling the test tubes in crushed ice. Nonphagocytosed bacteria were removed by differential centrifugation followed by two washes of the cells. Morphological studies with lysostaphin confirmed the intracellular localization of approximately 70% of the bacteria after this procedure. The antibacterial activity of penicillin G was assessed by incubation of monocytes containing ingested S. aureus with the drug at 37 C, and determination of the number of viable cell-associated bacteria was assessed microbiologically at various intervals. Inactivation of the intracellular killing mechanism of the monocytes by omission of serum during incubation allowed measurement of the effect of penicillin G apart from the bactericidal activity of the cells. The effect of penicillin G on nonphagocytosed bacteria was assessed after incubation of preopsonized S. aureus in medium with the drug. The results show that the effect of penicillin G on intracellular S. aureus is two to seven times stronger than the effect on nonphagocytosed S. aureus.
针对 b 型流感嗜血杆菌的抗生素的细胞摄取和细胞内活性。
DOI：
10.1093/infdis/145.2.152
发表时间：
1982
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Jacobs,RF;Wilson,CB;Laxton,JG;Haas,JE;Smith,AL
通讯作者：
Smith,AL