Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle
Fission yeast orb6, a ser/thr protein kinase related to mammalian rho kinase and myotonic dystrophy kinase, is required for maintenance of cell polarity and coordinates cell morphogenesis with the cell cycle
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DOI：
10.1073/pnas.95.13.7526
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发表时间：
1998-06-23
影响因子：
11.1
通讯作者：
Nurse, P
中科院分区：
文献类型：
--
作者：
Verde, F;Wiley, DJ;Nurse, P
协调细胞形态发生与细胞周期的分子机制在很大程度上仍然未知。我们研究了分裂酵母中的这一过程，其中极化细胞生长的变化与细胞周期进程相结合。orb 6基因在间期期间需要维持细胞极性，并编码丝氨酸/苏氨酸蛋白激酶，属于强直性肌营养不良激酶/cot 1/疣家族。Orb 6蛋白水平的降低导致极化细胞形状的丧失和有丝分裂的推进，而Orb 6水平的增加通过影响p34（cdc 2）有丝分裂激酶来维持极化生长并延迟有丝分裂。因此，Orb 6蛋白激酶在有丝分裂开始的间期协调细胞极性的维持，orb 6与编码Pak 1/Shk 1蛋白激酶的orb 2遗传相互作用，Ras 1和Cdc 42依赖性信号通路的一个组成部分。我们的研究结果表明，Orb 6可能作用于Pak 1/Shk 1的下游，形成协调细胞形态发生与细胞周期进展的途径的一部分。
The molecular mechanisms that coordinate cell morphogenesis with the cell cycle remain largely unknown. We have investigated this process in fission yeast where changes in polarized cell growth are coupled with cell cycle progression, The orb6 gene is required during interphase to maintain cell polarity and encodes a serine/threonine protein kinase, belonging to the myotonic dystrophy kinase/ cot1/warts family. A decrease in Orb6 protein levels leads to loss of polarized cell shape and to mitotic advance, whereas an increase in Orb6 levels maintains polarized growth and delays mitosis by affecting the p34(cdc2) mitotic kinase, Thus the Orb6 protein kinase coordinates maintenance of cell polarity during interphase with the onset of mitosis, orb6 interacts genetically with orb2, which encodes the Pak1/Shk1 protein kinase, a component of the Ras1 and Cdc42-dependent signaling pathway. Our results suggest that Orb6 may act downstream of Pak1/Shk1, forming part of a pathway coordinating cell morphogenesis with progression through the cell cycle.