Amplified FRET Nanoflares: An Endogenous mRNA-Powered Nanomachine for Intracellular MicroRNA Imaging
Amplified FRET Nanoflares: An Endogenous mRNA-Powered Nanomachine for Intracellular MicroRNA Imaging
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放大的 FRET 纳米耀斑：一种内源性 mRNA 驱动的纳米机器，用于细胞内 microRNA 成像
DOI：
10.1002/anie.202008245
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发表时间：
2020-08-31
影响因子：
16.6
通讯作者：
Wang, Kemin
中科院分区：
文献类型：
--
作者：
Li, Jing;Wang, Jiaoli;Wang, Kemin
对活细胞中生物分子的检测具有重要价值。然而，用于活细胞中低丰度靶标成像的探针受到一对一信号触发模型的限制。在这里，我们介绍了扩增FRET nanoflare的概念，它采用高丰度的内源性mRNA作为燃料链，以放大低丰度细胞内miRNA的检测。据我们所知，这是第一个报告的内源性mRNA驱动的纳米机器细胞内分子检测。我们通过实验证明了纳米机器的机制，并证明了其特异性和灵敏度。所提出的放大FRET nanoflare可以作为一种优秀的细胞内分子检测策略，是有前途的生物和医学应用。
It is of great value to detect biological molecules in live cells. However, probes for imaging low-abundance targets in live cells are limited by the one-to-one signal-triggered model. Here, we introduce the concept of the amplified FRET nanoflare, which employs high-abundance endogenous mRNA as fuel strands to amplify the detection of low abundance intracellular miRNA. As far as we know, this is the first report of an endogenous mRNA-powered nanomachine for intracellular molecular detection. We experimentally prove the mechanism of the nanomachine and demonstrate its specificity and sensitivity. The proposed amplified FRET nanoflare can act as an excellent intracellular molecular detection strategy that is promising for biological and medical applications.