Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.
Revealing the Specificity of Human H1 Influenza A Viruses to Complex N-Glycans.
复制标题
DOI：
10.1021/jacsau.2c00664
复制
发表时间：
2023-03-27
期刊：
影响因子：
8
通讯作者：
de Vries, Robert P
中科院分区：
文献类型：
--
作者：
Canales, Angeles;Sastre, Javier;Orduna, Jose M;Spruit, Cindy M;Perez-Castells, Javier;Dominguez, Gema;Bouwman, Kim M;van der Woude, Roosmarijn;Canada, Francisco Javier;Nycholat, Corwin M;Paulson, James C;Boons, Geert-Jan;Jimenez-Barbero, Jesus;de Vries, Robert P
流感病毒感染仍然是对人类健康的威胁，因为病毒血凝素不断漂移，逃避感染和疫苗诱导的抗体反应。来自不同病毒的病毒血凝素在聚糖识别中显示出变异性。在这种情况下，最近的H3 N2病毒对具有至少三个N-乙酰乳糖胺单元的α 2，6唾液酸化分支N-聚糖（tri-LacNAc）具有特异性。在这项工作中，我们将聚糖阵列和组织结合分析与核磁共振实验相结合，以表征H1变体家族的聚糖特异性，包括2009年大流行爆发的原因。我们还分析了一种工程化的H6 N1突变体，以了解对三-LacNAc基序的偏好是否可能是人类型受体适应病毒的普遍趋势。此外，我们开发了一种新的NMR方法来进行具有相似组成和不同长度的聚糖之间的竞争实验。我们的研究结果指出，大流行性H1病毒不同于以前的季节性H1病毒的严格偏好最低限度的二-LacNAc结构基序。
Influenza virus infection remains a threat to human health since viral hemagglutinins are constantly drifting, escaping infection and vaccine-induced antibody responses. Viral hemagglutinins from different viruses display variability in glycan recognition. In this context, recent H3N2 viruses have specificity for α2,6 sialylated branched N-glycans with at least three N-acetyllactosamine units (tri-LacNAc). In this work, we combined glycan arrays and tissue binding analyses with nuclear magnetic resonance experiments to characterize the glycan specificity of a family of H1 variants, including the one responsible for the 2009 pandemic outbreak. We also analyzed one engineered H6N1 mutant to understand if the preference for tri-LacNAc motifs could be a general trend in human-type receptor-adapted viruses. In addition, we developed a new NMR approach to perform competition experiments between glycans with similar compositions and different lengths. Our results point out that pandemic H1 viruses differ from previous seasonal H1 viruses by a strict preference for a minimum of di-LacNAc structural motifs.
影响因子：
5.6
作者：
Vasile F;Panigada M;Siccardi A;Potenza D;Tiana G
通讯作者：
Tiana G