Cocaine self-administration in mice with forebrain knock-down of trpc5 ion channels.
Cocaine self-administration in mice with forebrain knock-down of trpc5 ion channels.
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DOI：
10.12688/f1000research.2-53.v1
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Cooper DC
中科院分区：
文献类型：
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作者：
Pomrenze MB;Baratta MV;Rasmus KC;Cadle BA;Nakamura S;Birnbaumer L;Cooper DC
典型瞬时受体电位(TRPC)通道是一类非选择性阳离子通道，参与细胞内钙离子调节和树突生长，在调节神经元兴奋性方面起着至关重要的作用。TRPC5通道a)是成年啮齿动物大脑中最常见的两个TRPC通道之一；b)密集表达于前额叶皮质(PFC)的深层锥体神经元；c)调节工作记忆和注意力所需的神经元持续活动。为了评价TRPC5在动机/奖励相关行为中的因果作用，建立了条件性前脑TRPC5基因敲除(TRPC5-KD)小鼠，并对其进行了鼻戳可卡因的训练。在这里，我们提供了一个数据集，其中包含野生型(WT)和TRPC5-KD小鼠自我注射生理盐水或可卡因的前6天。此外，我们还提供了一个数据集，显示了两组达到相似水平的可卡因自我给药后对可卡因的剂量反应。与WT小鼠相比，TRPC5-KD小鼠在没有事先操作训练的情况下，在可卡因测试的第一天表现出明显的自我给药增加。WT小鼠和TRPC5-KD小鼠在训练第1天的生理盐水反应无明显差异。在达到类似水平的可卡因摄入量几天后，两组人对可卡因表现出类似的剂量反应敏感性。
Canonical transient receptor potential (TRPC) channels are a family of non-selective cation channels that play a crucial role in modulating neuronal excitability due to their involvement in intracellular Ca2+ regulation and dendritic growth. TRPC5 channels a) are one of the two most prevalent TRPC channels in the adult rodent brain; b) are densely expressed in deep layer pyramidal neurons of the prefrontal cortex (PFC); and c) modulate neuronal persistent activity necessary for working memory and attention. In order to evaluate the causal role of TRPC5 in motivation/reward-related behaviors, conditional forebrain TRPC5 knock-down (trpc5-KD) mice were generated and trained to nose-poke for intravenous cocaine. Here we present a data set containing the first 6 days of saline or cocaine self-administration in wild type (WT) and trpc5-KD mice. In addition, we also present a data set showing the dose-response to cocaine after both groups had achieved similar levels of cocaine self-administration. Compared to WT mice, trpc5-KD mice exhibited an apparent increase in self-administration on the first day of cocaine testing without prior operant training. There were no apparent differences between WT and trpc5-KD mice for saline responding on the first day of training. Both groups showed similar dose-response sensitivity to cocaine after several days of achieving similar levels of cocaine intake.