Olig transcription factors are expressed in oligodendrocyte and neuronal cells in human fetal CNS
Olig transcription factors are expressed in oligodendrocyte and neuronal cells in human fetal CNS
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DOI：
10.1523/jneurosci.2324-05.2005
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发表时间：
2005-11-02
影响因子：
5.3
通讯作者：
Zecevic, N
中科院分区：
文献类型：
--
作者：
Jakovcevski, I;Zecevic, N
转录因子Olig 1和Olig 2与脊椎动物神经系统中少突胶质细胞（oligodendrocyte，OL）谱系的发育密切相关，但其在人类中枢神经系统发育中的作用却知之甚少。为了检验它们有助于人类初始OL特化的假设，我们研究了Olig 1和Olig 2在5 - 24孕周（GW）的人类胎儿中的表达。这两种转录因子都存在于腹侧神经上皮在5 GW，少突分化前几周的轮廓分明的区域。Olig 1和Olig 2沿着神经元轴表达的空间差异表明它们指定了不同的祖细胞亚群。Olig 1从基底前脑到后脑分布在喙部，而Olig 2也分布在腹侧脊髓中。此外，在5 GW，Olig 1与波形蛋白共表达，Olig 2与神经元标记物微管相关蛋白2共表达。随着15 GW的发展，这两种蛋白质都存在于整个脊髓和脑室脑室下区的神经节隆起，而在妊娠中期（20 GW），他们也表达在端脑增生区和新兴的白色物质。双标记研究显示，早期OL祖细胞和放射状胶质细胞表达Olig 1，而Olig 2主要定位于成熟OL和神经祖细胞和成熟神经元的子集。因此，Olig 1和Olig 2转录因子在人类中枢神经系统是重要的，不仅为分化的OL谱系，但他们也可能有一个作用，在神经细胞的规范。
The transcription factors Olig1 and Olig2 are closely associated with the development of oligodendrocyte (OL) lineage in the vertebrate nervous system, but little is known about their role in the human developing CNS. To test the hypothesis that they contribute to initial OL specification in humans, we studied the expression of Olig1 and Olig2 in human fetuses at 5 - 24 gestational weeks (GW). Both transcription factors were present in well outlined regions of the ventral neuroepithelium at 5 GW, several weeks before oligodendrogenesis. Spatial differences in the expression of Olig1 and Olig2 along the neuronal axis suggest that they specify different subpopulations of progenitor cells. Olig1 was distributed rostrally, from the basal forebrain to the hindbrain, whereas Olig2 was also found in the ventral spinal cord. Furthermore, at 5 GW, Olig1 was coexpressed with vimentin, and Olig2 was coexpressed with a neuronal marker, microtubule-associated protein 2. With the progression of development at 15 GW, both proteins were present throughout the spinal cord and the ventricular-subventricular zone of the ganglionic eminences, whereas at midgestation ( 20 GW), they were also expressed in the telencephalic proliferative zones and the emerging white matter. Double-labeling studies revealed that early OL progenitor cells and radial glia expressed Olig1, whereas Olig2 was localized predominantly in mature OLs and a subset of neural progenitor cells and mature neurons. Thus, Olig1 and Olig2 transcription factors in the human CNS are important not only for differentiation of the OL lineage, but they may also have a role in neural cell specification.