Chromatin interaction networks and higher order architectures of eukaryotic genomes.
Chromatin interaction networks and higher order architectures of eukaryotic genomes.
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染色质相互作用网络和真核基因组的高阶结构。
DOI：
10.1002/jcb.23155
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发表时间：
2011
影响因子：
4
通讯作者：
Ruan,Yijun
中科院分区：
文献类型：
--
作者：
SinghSandhu,Kuljeet;Li,Guoliang;Sung,Wing-Kin;Ruan,Yijun
真核生物基因组不仅是线性的，而且是空间的，组织成非随机结构。虽然基因的线性组织和它们的表观遗传描述符的特点，其空间组织的相关性是最近才开始展开。越来越多的人认识到，远距离基因组元件之间的物理相互作用可以作为真核基因组调控的重要手段。随着基于邻位连接的技术与下一代测序相结合的出现，现在可以以高分辨率探索全基因组染色质相互作用。关于全基因组染色质相互作用的新数据表明，距离较远的基因不是独立的实体，而是以广泛的方式相互交叉，支持“染色质相互作用网络”的概念。此外，这些数据还将该领域推进到“三维（3D）染色质结构和动力学”，这将使分子生物学家能够探索基因组的时空调控。在这篇文章中，我们介绍了基因组从一维（1D，线性）到二维（2D，网络）再到三维（3D，架构）的逐步拓扑变换，并讨论了这种变换如何促进我们对基因组生物学的理解。J.细胞。112：2218-2221，2011中。© 2011 Wiley利斯公司
Eukaryotic genome is, not only linearly but also spatially, organized into non‐random architecture. Though the linear organization of genes and their epigenetic descriptors are well characterized, the relevance of their spatial organization is beginning to unfold only recently. It is increasingly being recognized that physical interactions among distant genomic elements could serve as an important mean to eukaryotic genome regulation. With the advent of proximity ligation based techniques coupled with next generation sequencing, it is now possible to explore whole genome chromatin interactions at high resolution. Emerging data on genome‐wide chromatin interactions suggest that distantly located genes are not independent entities and instead cross‐talk with each other in an extensive manner, supporting the notion of “chromatin interaction networks”. Moreover, the data also advance the field to “3‐dimensional (3D) chromatin structure and dynamics”, which would enable molecular biologists to explore the spatiotemporal regulation of genome. In this article, we introduce a stepwise topological transformation of genome from 1‐dimension (1D, linear) to 2‐dimension (2D, networks) to 3‐dimension (3D, architecture) and discuss how such transformations could advance our understanding of genome biology. J. Cell. Biochem. 112: 2218–2221, 2011. © 2011 Wiley‐Liss, Inc.