A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.)
A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.)
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DOI：
10.1007/s11295-009-0247-4
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发表时间：
2010-02-01
影响因子：
2.4
通讯作者：
Seymour, Graham B.
中科院分区：
文献类型：
--
作者：
Cevik, Volkan;Ryder, Carol D.;Seymour, Graham B.
FRUITFULL（FUL）和SHATTERPROOF（SHP）基因参与调节拟南芥果实发育和开裂。我们测试的假设，这类基因也参与调节肉质果实的发育，通过探索苹果（苹果）基因库内的遗传和表型变异。我们分离并鉴定了两个候选的正向FUL样基因MdMADS2.1和MdMADS2.2的基因组序列。这些被映射使用参考群体'Prima x Fiesta'到苹果连锁群LG 14和LG 06上的基因座。另外一个MADS盒基因MdMADS 14与拟南芥SHATTERPROOF 1/2基因具有高度的氨基酸同一性，并被定位到苹果属连锁群LG 09。利用混合模型方差分析对“Prima x Fiesta”后代的果肉硬度与MdMADS 2.1、MdMADS 2.2和MdMADS 14位点进行了关联分析。MdMADS2.1与果肉硬度呈显著相关（P < 0.01）。使用基于病例对照群体的遗传关联方法获得了MdMADS2.1和果肉硬度之间关联的进一步证据。为此，在MdMADS2.1的3' UTR中的多态重复序列（AT）（n）被用作基因座特异性标记以筛选168个苹果种质，其中果实质地属性的历史评估是可用的。结果表明，MdMADS2.1与果肉硬度在等位基因（chi（2）= 34，df = 9，P < 0.001）和基因型（chi（2）= 57，df = 32，P < 0.01）水平上均存在显著相关。
The FRUITFULL (FUL) and SHATTERPROOF (SHP) genes are involved in regulating fruit development and dehiscence in Arabidopsis. We tested the hypothesis that this class of genes are also involved in regulating the development of fleshy fruits, by exploring genetic and phenotypic variation within the apple (Malus domestica) gene pool. We isolated and characterised the genomic sequences of two candidate orthologous FUL-like genes, MdMADS2.1 and MdMADS2.2. These were mapped using the reference population 'Prima x Fiesta' to loci on Malus linkage groups LG14 and LG06, respectively. An additional MADS-box gene, MdMADS14, shares high amino acid identity with the Arabidopsis SHATTERPROOF1/2 genes and was mapped to Malus linkage group LG09. Association analysis between quantitative fruit flesh firmness estimates of 'Prima x Fiesta' progeny and the MdMADS2.1, MdMADS2.2 and MdMADS14 loci was carried out using a mixed model analysis of variance. This revealed a significant association (P < 0.01) between MdMADS2.1 and fruit flesh firmness. Further evidence for the association between MdMADS2.1 and fruit flesh firmness was obtained using a case-control population-based genetic association approach. For this, a polymorphic repeat, (AT)(n), in the 3' UTR of MdMADS2.1 was used as a locus-specific marker to screen 168 apple accessions for which historical assessments of fruit texture attributes were available. This analysis revealed a significant association between the MdMADS2.1 and fruit flesh firmness at both allelic (chi (2) = 34, df = 9, P < 0.001) and genotypic (chi (2) = 57, df = 32, P < 0.01) levels.