STRUCTURAL ORGANIZATION OF THE MAXICIRCLE VARIABLE REGION OF TRYPANOSOMA-BRUCEI - IDENTIFICATION OF POTENTIAL REPLICATION ORIGINS AND TOPOISOMERASE-II BINDING-SITES
STRUCTURAL ORGANIZATION OF THE MAXICIRCLE VARIABLE REGION OF TRYPANOSOMA-BRUCEI - IDENTIFICATION OF POTENTIAL REPLICATION ORIGINS AND TOPOISOMERASE-II BINDING-SITES
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DOI：
10.1093/nar/21.3.687
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发表时间：
1993-02-11
影响因子：
14.9
通讯作者：
STUART, KD
中科院分区：
文献类型：
--
作者：
MYLER, PJ;GLICK, D;STUART, KD
寄生原生动物布鲁氏锥虫（Trypanosoma bruei）是线粒体基因组的一个组成部分，在ND5和12S rRNA基因之间的可变区（VR）定位的分离物中，其最大环的大小存在差异。我们在这里给出了整个区域的核苷酸序列，从而完成了最大环基因组的序列。我们还从大部分VR中发现了大小不一的转录本。VR有三个不同的部分，每个部分都有独特的重复序列。两个部分的重复序列都很短，并且高度重复；种内大小变化发生在这一区域内。第三部分包含非重复序列和12S rRNA基因上游的大量重复。第一节中的两个重复单元包含一个序列，该序列与微环的DNA复制起源具有同源性。该区域还包含与拓扑异构酶II结合和裂解位点同源的序列。这些发现表明VR在最大环DNA复制中的作用。
The maxicircle of the parasitic protozoan Trypanosoma brucei, one component of the mitochondrial genome, has size differences among isolates that localize to the variable region (VR) between the ND5 and 12S rRNA genes. We present here the nucleotide sequence of this entire region, thus completing the sequence of the maxicircle genome. We also find heterogeneously sized transcripts from throughout most of the VR. The VR has three distinct sections, each with characteristic repeated sequences. The repeated sequences in two sections are short and highly reiterated; the intraspecies size variation occurs within this region. The third section contains non-repetitive sequences and a large duplication immediately upstream of the 12S rRNA gene. Two repeat units within section I contain a sequence that has homology to the DNA replication origin of minicircles. This region also contains sequences with homology to topoisomerase II binding and cleavage sites. These findings suggest a role for the VR in DNA replication of the maxicircle.