Identification of two major histocompatibility (MH) class II A genes and their association to Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)
Identification of two major histocompatibility (MH) class II A genes and their association to Vibrio anguillarum infection in half-smooth tongue sole (Cynoglossus semilaevis)
复制标题
两个主要组织相容性 (MH) II A 类基因的鉴定及其与半滑舌鳎 (Cynoglossus semilaevis) 弧菌感染的关联
DOI：
10.1007/s11802-012-1802-4
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发表时间：
2012-03-01
影响因子：
1.6
通讯作者：
Yu Haiyang
中科院分区：
文献类型：
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作者：
Li Chunmei;Wang Xubo;Yu Haiyang
主要组织相容性复合体II类抗原在脊椎动物免疫系统中起重要作用。本研究利用RACE-PCR方法从半光滑舌鳎（Cynoglossus semilaevis）中合成了IIAgene类的全cDNA序列，并对其开放阅读框（ORF）多态性进行了研究。cDNA全长992 bp，其中ORF全长717 bp。25个等位基因被鉴定出来，并根据特定位置的特定核苷酸/氨基酸聚类成两个不同的群体。11个等位基因属于cyse - da， 14个等位基因属于cyse - dba。在1个个体中观察到4个cyse - da等位基因，在3个检测个体中每个个体中观察到3 ~ 5个cyse - dba等位基因，说明每个基因中至少存在2个位点。此外，为了研究这些等位基因在抗感染中的作用，我们对200个个体进行了腹腔注射，并选择前20个死亡个体和20个存活个体进行基因型分析。在40个个体中鉴定出56个等位基因。29个等位基因属于cyse - daa，另外27个等位基因属于cyse - dba。选择18个等位基因，研究其在抗感染中的功能。等位基因Cyse-DAA*0201、Cyse-DAA*1101、Cyse-DBA*0401、Cyse-DBA*1102、Cyse-DBA*1801和Cyse-DBA*2201仅在存活个体中发现，而等位基因Cyse-DAA*0901、Cyse-DBA*1101和Cyse-DBA*1401在死亡个体中更常见。本研究证实了半光滑舌底中两个IIAgenes类等位基因的存在和多态性，以及IIAgenes类等位基因与疾病易感/抗性的关系。
Major histocompatibility complex class II antigens are important in vertebrate immune system. In the present study, the full cDNA sequence of class IIAgene was synthesized by RACE-PCR from half-smooth tongue sole (Cynoglossus semilaevis), and its open reading frame (ORF) polymorphism was studied. The whole cDNA sequence was 992 bp in length, including the ORF with 717 bp. Twenty-five alleles were identified and clustered into two distinct groups according to the specific nucleotides/ amino acids in specific positions. Eleven alleles belonged toCyse-DAAwhile fourteen alleles belonged toCyse-DBA. FourCyse-DAAalleles were observed in one individual, and three to fiveCyse-DBAalleles were observed in each of the three detected individuals, which indicated that at least two loci existed in each gene. Moreover, in order to study the function of the alleles in resistance to infection, 200 individuals were intraperitoneally injected withVibrio anguillarumand the first 20 dead individuals and 20 surviving ones were selected for genotype analysis. Fifty-six alleles were identified among the 40 individuals. Twenty-nine alleles belonged toCyse-DAAand the other 27 alleles belonged toCyse-DBA. Eighteen alleles were selected for studying their function in resistance to infection. AllelesCyse-DAA*0201,Cyse-DAA*1101,Cyse-DBA*0401,Cyse-DBA*1102,Cyse-DBA*1801 andCyse-DBA*2201 were identified only in surviving individuals, while allelesCyse-DAA*0901,Cyse-DBA*1101 andCyse-DBA*1401 occurred more frequently in dead individuals. This study confirmed the existence and polymorphism of two class IIAgenes as well as the relationship between alleles of class IIAgenes and disease susceptibility/ resistance in half-smooth tongue sole.