Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs.
Second harmonic generation imaging of collagen scaffolds within the alveolar ducts of healthy and emphysematous mouse lungs.
复制标题
健康和肺气肿小鼠肺肺泡管内胶原支架的二次谐波成像。
DOI：
10.1007/s00418-020-01959-6
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发表时间：
2021
影响因子：
2.3
通讯作者：
Hackett,TillieL
中科院分区：
文献类型：
--
作者：
Mostaco-Guidolin,LeilaB;Loube,Jeffrey;Barlow,Aaron;Osei,EmmanuelT;Vasilescu,DragoșM;Hsieh,Aileen;Fouadi,May;Young,Christine;Scott,AlanL;Mitzner,Wayne;Hackett,TillieL
肺泡管连接到从脏层胸膜延伸到小叶间隔的外周间隔纤维，并锚定到小气道中的轴向纤维。这些轴向纤维和间隔纤维一起构成纤维连续体，其在整个肺中提供张力和完整性。基于观察结果，即与胸膜下肺泡相关的肺泡导管垂直于脏层胸膜，并且彼此平行，本研究的目的是研究健康对照和弹性蛋白酶诱导的肺气肿小鼠肺中胸膜下肺泡导管内胶原纤维组织的性质。利用三维二次谐波成像技术，可以在透明小鼠肺中观察到原纤维胶原纤维的结构排列。在健康对照肺中，肺泡口内的纤维状胶原纤维在肺泡管内形成卷曲的胶原结构。在弹性蛋白酶处理的肺气肿中，存在纤维状胶原纤维的损失（p< 0.01）和胶原结构组织的破坏，如通过纤维状胶原长度（p< 0.01）和熵（p< 0.01）测量的。与健康对照的肺泡管相比，与肺气肿肺相比，细胞面积（nm 2，p< 0.001）和具有胞质颗粒的细胞面积（nm 2，p< 0.001）显著增加。这些结果与肺气肿肺中肺泡表面的进行性损失和弹性回缩的主要贡献者之一是胶原支架的结构完整性的损失的想法一致，胶原支架保持对细胞存活和肺功能重要的空间关系。
The alveolar ducts are connected to peripheral septal fibers which extend from the visceral pleura into interlobular septa, and are anchored to axial fibers in the small airways. Together these axial and septal fibers constitute a fiber continuum that provides tension and integrity throughout the lung. Building on the observations that alveolar ducts associated with sub-pleural alveoli are orientated perpendicular to the visceral pleura, and in parallel to each other, the goal of the present study was to investigate the nature of the collagen fiber organization within sub-pleural alveolar ducts in healthy control and elastase-induced emphysema murine lungs. Employing three-dimensional second harmonic generation imaging, the structural arrangement of fibrilar collagen fibers could be visualized in cleared murine lungs. In healthy control lungs, fibrilar collagen fibers within alveolar mouths formed the coiled collagen structure within the alveolar duct. In the elastase-treated emphysema lungs, there was loss of fibrilar collagen fibers (p< 0.01) and disruption of collagens structural organization as measured by the fibrillar collagen length (p< 0.01) and entropy (p< 0.01). Compared to the alveolar ducts from healthy controls, there was a significant increase in the area of cells (nm2,p< 0.001), and area of cells with cytoplasmic granules (nm2,p< 0.001) compared to emphysematous lungs. These results are consistent with the idea that one of the major contributors to the progressive loss of alveolar surfaces and elastic recoil in the emphysematous lung is loss of the structural integrity of the collagen scaffold that maintains the spatial relationships important for cell survival and lung function.